Sequences from confirmation amplicon


Confirmation sequences for line 060E03 and hit At1g64190 are shown.
Line ID 060E03
Locus At1g64190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23825580
Primers used for PCR 58op - 8474, show primer details
   
Sequence Name 42-K114782-0022-060-E03C-58op
GenBank Accession LN504564 [GenBank]
Sequencing primer gene specific primer, 58op: 5'-GTTGGAACTTGAATTTTGGTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114782-0022-060-E03C-58op
TAGAGCGGTTTCTTGGATAGGATCAGAAAGCTTACCAGAGGAACCCGGACTTGGCTAGTC
TTGTGGTTGATCCTGAGTTTGCTAAAGAGATGGTTCAGAGACAAGCTGCTTGGAGAAGAG
TTGTTGGTTTGGCTGTTTCTGCTGGGATTAGCACGCCTGGAATGTGTGCTAGTCTTGCGT
ATTTCGATACTTATAGACGTGCTAGATTGCCGGCGAATCTGGTTCAGGCGCAGAGAGATC
TCTTTGGAGCTCACACTTATGAGAGAACCGATCGTCCTGGTGCGTACCATACTGATCAGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 42-K114769-0022-060-E03C-8409
GenBank Accession LN504563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114769-0022-060-E03C-8409
ACCAGAATTTGCCGGCAATCTAGCACGTCTATAAGTATCGAAATACGCAAGACTAGCACA
CATTCCAGGCGTGCTAATCCCAGCAGAAACAGCCAAACCAACAACTCTTCTCCAAGCAGC
TTGTCTCTGAACCATCTCTTTAGCAAACTCAGGATCAACCACAAGACTAGCCAAGTCCGG
GTTCCTCTGGTAAGCTTTCTTGATCCTATCCAAGAAAACCGCTCTTATAATACAACCACC
TTTCCAAATCCTAGCCAATTCACCA

Last Updated on 06.12.2018 16:11