Sequences from confirmation amplicon


Confirmation sequences for line 060G05 and hit At3g54300 are shown.
Line ID 060G05
Locus At3g54300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20109008
Primers used for PCR TU02 - 8474, show primer details
   
Sequence Name 13-K027352-022-060-G05C-8409
GenBank Accession LN491885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K027352-022-060-G05C-8409
TTTACAATTGAACAGGGGTCTGGAAATCTAATTCAAGCTGCGTGCAACTTCTTAGTTTCT
ATCAGACTTAGATACTAAAGTCCATTCTGTCACAGTTTTAAAAGTTGACAAAGGTCTGGA
AAGAAAACACAGTAATTCACAAACAATATAATTATACCAGATATAGGTTAATGAAAGAAT
GGTTGTGAGAAAAGCAATTGATACATTTATCCAGTAAAACTACCATGGAAGTAAACCTAT
TTATTTGGTGTCAAATATCTCTTCTAGAAAGTATTTGTTGTTCAGCATCTTTCCTACAGT
ACTTTTTTGTAATTGCAAAATCATGTGGATAAAAATGTTGTCACCTTAAACCAAAAAAGT
ACAAGATCTGCGTGCTACTGTTAATTAAAATGTCTAAGCTAGTTCATCTTTTCTTATAAC
GACAAAAGCATACCTTCTCAATGTTGTCCATCATGATCCCCTTGACCTCAGTTATTTGGG
CCTTCAACTTAGAAAGTTTGCTCATCTCTTCTGGATGGCTCATACAATACTGCATATGCT
CCTTTAGTATGGGCCTATTAGAGGGGAAAGAAAAGAATCAGAACACAGTTGCAATGCGAA
AC
   
Sequence Name 14-K027352-022-060-G05C-TU02
GenBank Accession LN492354 [GenBank]
Sequencing primer gene specific primer, TU02: 5'-TCTGATATCACTTCATTGTTTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027352-022-060-G05C-TU02
CCTCTATAGGCCCATACTAAAGGAGCATATGCAGTATTGTATGAGCCATCCAGAAGAGAT
GAGCAAACTTTCTAAGTTGAAGGCCCAAATAACTGAGGTCAAGGGGATCATGATGGACAA
CATTGAGAAGGTATGCTTTTGTCGTTATAAGAAAAGATGAACTAGCTTAGACATTTTAAT
TAACAGTAGCACGCAGATCTTGTACTTTTTTGGTTTAAGGTGACAACATTTTTATCCACA
TGATTTTGCAATTACAAAAAAGTACTGTAGGAAAGATGCTGAACAACAAATACTTTCTAG
AAGAGATATTTGACACCAAATAAATAGGTTTACTTCCATGGTAGTTTTACTGGATAAATG
TATCAATTGCTTTTCTCACAACCATTCTTTCATTAACCTATATCTGGTATAATTATATTG
TTTGTGAATTACTGTGTTTTCTTTCCAGACCTTTGTCAACTTTTAAAACTGTGACAGAAT
GGACTTTAGTATCTAAGTCTGATAGAAACTAAGAAGTTGCACGCAGCTTGAATTAGATTT
CCAGACCCCTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
T

Last Updated on 06.12.2018 16:11