Sequences from confirmation amplicon


Confirmation sequences for line 061B06 and hit At3g21610 are shown.
Line ID 061B06
Locus At3g21610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7609443
Primers used for PCR 05sj - 8474, show primer details
   
Sequence Name 25-K116239-0022-061-B06C-05sj
GenBank Accession LN497606 [GenBank]
Sequencing primer gene specific primer, 05sj: 5'-TGGTCATAGATATGGTTTCGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116239-0022-061-B06C-05sj
GAATGAGAGATTATATATATATATATAGAGAGAGAGAGAATGTTGAGAAGGCGTAAGCTT
AAACAATGACAAGTCTAACTAAGATCATCTATATATTACACAGATTTAACAATTTCCATT
TACTCCAATAATCTAGCGTCACTTGATATCGCTGAATCTATCTTAGTACCTGAAGGGTCT
ATGATTTCAAAAGAATTCGGGATTTGTTCTTGTGTAGAGAGACTGACTAGCTTGAGCTCC
TCATTAAGTAAGCTACCACGCATCCTAAAATCCCACCTGCTGCAACCTGTATTTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATA
   
Sequence Name 01-K116238-0022-061-B06C-8409
GenBank Accession LN486725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116238-0022-061-B06C-8409
GAATACAGGTTGCAGCAGGTGGGATTTTAGGATGCGTGGTAGCTTACTTAATGAGGAGCT
CAAGCTAGTCAGTCTCTCTACACAAGAACAAATCCCGAATTCTTTTGAAATCATAGACCC
TTCAGGTACTAAGATAGATTCAGCGATATCAAGTGACGCTAGATTATTGGAGTAAATGGA
AATTGTTAAATCTGTGTAATATATAGATGATCTTAGTTAGACTTGTCATTGTTTAAGCTT
ACGCCTTCTCAACATTCTCTCTCTCTCTATATATATATATATAATCTCTCTATTTCCTCT
TCCTCCTAATCATGATCAGTTTTATGCGAAACCATATCTATGACCAA

Last Updated on 06.12.2018 16:11