Sequences from confirmation amplicon


Confirmation sequences for line 062C02 and hit At4g18350 are shown.
Line ID 062C02
Locus At4g18350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10142668
Primers used for PCR 07kh - 8474, show primer details
   
Sequence Name 64-K113595-0022-062-C02C-07kh
GenBank Accession LN511244 [GenBank]
Sequencing primer gene specific primer, 07kh: 5'-GGTTATTTTAACTGCGTGAACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K113595-0022-062-C02C-07kh
TGGCTCACATCGGATTCGCGCCGTTACGGATATAACACCGTCAATGCAGTCAGGGATTGT
TCCTTCGACGGTGAGGTTTCGCCGGACGGAAGATTCCGGTACCGGGGAATAATTCCCGGC
AATCTGAACACGTGGATCAGCGGTTTTGGGAAGTGGAGAATCTTGCTCGTGTGAGATTAA
TGCACGCTCAGCCGCGTCGATCGCAATCGCCGCCGCTTTCTGGAAGATGTTTAGCCGGAG
AGGATTATGCTGCGGCGGAGTAGCGGTGGTTCTGGGTGTAAATAATTGGCGTTTTTTGGT
TAATTCCGTTACGTCGATCTGCACCGTGCATTTAATAACCTTCGGTTGTCTTTTAATGGG
CCTAAAACCCAAATCAATTTGGGCTTGAGGCCATGTTTTAATACCACCACTCATCGGCAT
TGTAAGAAGAGAAACCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 22-K113594-0022-062-C02C-8409
GenBank Accession LN496196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113594-0022-062-C02C-8409
TCGGATGAGCCTGGTTTCTCTTCTTACAATGCCGATGAGTGGTGGTATTAAAACATGGCC
TCAAGCCCAAATTGATTTGGGTTTTAGGCCCATTAAAAGACAACCGAAGGTTATTAAATG
CACGGTGCAGATCGACGTAACGGAATTAACCAAAAAACGCCAATTATTTACACCCAGAAC
CACCGCTACTCCGCCGCAGCATAATCCTCTCCGGCTAAACATCTTCCAGAAAGCGGCGGC
GATTGCGATCGACGCGGCTGAGCGTGCATTAATCTCACACGAGCAAGATTCTCCACTTCC
CAAAACCGCTGATCCACGTGTTCAGATTGCCGGGAATTATTCCCCGGTACCGGAATCTTC
CGTCCGGCGAAACCTCACCGTCGAAGGAACAATCCCTGACTGCATTGACGGTGTTTATAT
CCGTAACGGCGCGAATCCGATGTTTGAGCCAACAGCTGGGCACCATTTATTCGACGGAGA
CGGAATGGTTCACGCAGTTAAAATAAC

Last Updated on 06.12.2018 16:11