Sequences from confirmation amplicon


Confirmation sequences for line 062H01 and hit At1g03365 are shown.
Line ID 062H01
Locus At1g03365 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:828143
Primers used for PCR MF06 - 8474, show primer details
   
Sequence Name 62-K031691-022-062-H01C-8409
GenBank Accession LN510668 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K031691-022-062-H01C-8409
AAATAGGACTTACAGCAAAGGCAGTGGCTATAAGGCGGCAAAGTTTGCATCAGTTGGCAG
CTTCCTTTTAGAGAAAAGAGTCAAATCATCTTCTGAATTTGTGCCAAGAAATTCTTCATC
AAAGATAACAGCAGAGATTGGAGTGAAAGTTTCATTAGCTGAGGATAGCGGCTGTTTTGT
AAGAAAGAACAGTAAGTTGGATTCACCTGTGGTAGTGGTAGATGCAAAGGGCTATATAAC
TGCCTTACCTGCTAGGTCGGTAAAATCTGCTTCAAAAAAAAAGACTGGTTCTGAGTCTGT
AACTCTGATACCATCAGCTTCAGAAAAGAAGT
   
Sequence Name 14-K031691-022-062-H01C-MF06
GenBank Accession LN492390 [GenBank]
Sequencing primer gene specific primer, MF06: 5'-AGACTTCTTTTCTGAAGCTGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031691-022-062-H01C-MF06
ACAGTCTTTTTTTTTGAGCAGATTTTACCGACCTAGCAGGTAAGGCAGTTATATAGCCCT
TTGCATCTACCACTACCACAGGTGAATCCAACTTACTGTTCTTTCTTACAAAACAGCCGC
TATCCTCAGCTAATGAAACTTTCACTCCAATCTCTGCTGTTATCTTTGATGAAGAATTTC
TTGGCACAAATTCAGAAGATGATTTGACTCTTTTCTCTAAAAGGAAGCTGCCAACTGATG
CAAACTTTGCCGCCTTATAGCCACTGCCTTTGCTGTAAGTCCTAATTTTTTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG
Primers used for PCR AG05 - 8474, show primer details
   
Sequence Name 50-K118809-0022-062-H01C-AG05
GenBank Accession LN507327 [GenBank]
Sequencing primer gene specific primer, AG05: 5'-TGTTTGAGGATTTACAACAGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118809-0022-062-H01C-AG05
GGAGGTCAGGCCGTCCCTATCAACAGTTGAAGCTATGTGGAGGCTTTTGATGTGCGACTT
GAATGTCTTGCAAGCCTTTGAAGCTGAAGGCGATGGATTGGTGAGTTCAAGTAAATTATC
TGATTCGGAATCTCTTGGTGCTGAATCTAATCCTCCAAAATCTAGTGACCCAGATAACCC
AAAGCCTCCCCAATCCGATCCTCAAAGCAATCGGAATGAGCCACTCAAATTTGGAAACTT
CCCAAATACACCGAACTCCAAAAAAACTCAGTCTAGTGGAACAACACCAGGAAAAGAAGT
ATGCTCGGGTAGCACTGTTTCTTGTCAAGGCATGAGAAGTACATCATTTACTCTAGTCTC
TGATGAAAAATTGGTATCTTGTCGAAAAGGCCGTACTAAAAAAGAGATAGCAATGCTTCA
ATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCACA
   
Sequence Name 18-K118808-0022-062-H01C-8409
GenBank Accession LN494526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118808-0022-062-H01C-8409
TCTTTTTAGTACGGCCTTTTCGACAAGATACCAATTTTTCATCAGAGACTAGAGTAAATG
ATGTACTTCTCATGCCTTGACAAGAAACAGTGCTACCCGAGCATACTTCTTTTCCTGGTG
TTGTTCCACTAGACTGAGTTTTTTTGGAGTTCGGTGTATTTGGGAAGTTTCCAAATTTGA
GTGGCTCATTCCGATTGCTTTGAGGATCGGATTGGGGAGGCTTTGGGTTATCTGGGTCAC
TAGATTTTGGAGGATTAGATTCAGCACCAAGAGATTCCGAATCAGATAATTTACTTGAAC
TCACCAATCCATCGCCTTCAGCTTCAAAGGCTTGCAAGACATTCAAGTCGCACATCAAAA
GCCTCCACATAGCTTCAACTGTTGATAGGGACGGCCTGACCTCCTTAATAAGACTTATCA
TTTCTACCAACGTATAGGAAACAAGCTGTTGTAATCNCTCAAAC

Last Updated on 06.12.2018 16:11