Sequences from confirmation amplicon


Confirmation sequences for line 064E04 and hit At1g43610 are shown.
Line ID 064E04
Locus At1g43610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16425270
Primers used for PCR 03oc - 8474, show primer details
   
Sequence Name 42-K114864-0022-064-E04C-03oc
GenBank Accession LN504566 [GenBank]
Sequencing primer gene specific primer, 03oc: 5'-TCTGCAACTCAATTCATTTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114864-0022-064-E04C-03oc
ATATAGATTCTTTTGCCGTTGCATAAAATGGATGTAATAAAATTATTGTTATGGGTTTGT
TCATCTTTATAAGTTTGTGGTCCAATGAGAATGACTTTGAAGGATGATTAATAAAATTCA
AAACTATATTTATTCAAGAGCTTGTTACAAAGCTAGAAACATTCTAATCTCTAAGTAGCT
TTAAGAGTACAAAACTCTGAATCAAATCTACTTACTGTCACTCAGTAGCAGCGTTGTCTA
CAAGGTATCAATAGCCTCAAATCTTTAAAACCTAAATCCTCTTCAAATCTTTTGTGATAT
ATTGACCGAGAAATCCAATGGAGATATCACATCAATCCATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGC
   
Sequence Name 02-K114863-0022-064-E04C-8409
GenBank Accession LN487147 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114863-0022-064-E04C-8409
GATTTCTCGGTCATATATCACAAAAGATTTGAAGAGGATTTAGGTTTTAAAGATTTGAGG
CTATTGATACCTTGTAGACAACGCTGCTACTGAGTGACAGTAAGTAGATTTGATTCAGAG
TTTTGTACTCTTAAAGCTACTTAGAGATTAGAATGTTTCTAGCTTTGTAACAAGCTCTTG
AATAAATATAGTTTTGAATTTTATTAATCATCCTTCAAAGTCATTCTCATTGGACCACAA
ACTTATAAAGATGAACAAACCCATAACAATAATTTTATTACATCCATTTTATGCAACTGT
GCAAAAGAAATCTAATTATATTCCTGACCATTGAATGAAATGAATTGAGTTGCAG

Last Updated on 06.12.2018 16:11