Sequences from confirmation amplicon


Confirmation sequences for line 065A08 and hit At2g17440 are shown.
Line ID 065A08
Locus At2g17440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7572072
Primers used for PCR 11eq - 8474, show primer details
   
Sequence Name 17-K110827-0022-065-A08C-11eq
GenBank Accession LN493901 [GenBank]
Sequencing primer gene specific primer, 11eq: 5'-TATAGACTCAGGGAGCTGACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K110827-0022-065-A08C-11eq
CAAGAATAAGCCCTCCTATGGTTGCCGGTAGTACCATGATACAATTCTCAGACAGATCTA
GCCTGACAAGACTCGATAACTTGCCTAGAGAATCCGGAAGCCATTCGAGCTGATCCATCA
GCCTGTGCTGCAAATTTAGCTCTTGAGTAGCTTTCTTGGCAGATACCTCAATCAAACTAG
CAAGTTTTATGAGACTCAACTTCTCCCCATCATTGCCTACACACACATAATAATAATTCC
ACTACAATTAGAGACAAACAGACTCCGTTGGAACACATTCTAAACACATGAAATCTCAAG
CTTAGATTCCATTGAAAAAGCAAACTAACCACATGAAAAGCACTATATAAATCTTAACCT
CAGATTCTATCAAAAAGTTGAAACATTTCAATTCCAAACACTTTTTTCCATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 01-K110826-0022-065-A08C-8409
GenBank Accession LN486626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K110826-0022-065-A08C-8409
GTGTTTGGAATTGAAATGTTTCAACTTTTTGATAGAATCTGAGGTTAAGATTTATATAGT
GCTTTTCATGTGGTTAGTTTGCTTTTTCAATGGAATCTAAGCTTGAGATTTCATGTGTTT
AGAATGTGTTCCAACGGAGTCTGTTTGTCTCTAATTGTAGTGGAATTATTATTATGTGTG
TGTAGGCAATGATGGGGAGAAGTTGAGTCTCATAAAACTTGCTAGTTTGATTGAGGTATC
TGCCAAGAAAGCTACTCAAGAGCTAAATTTGCAGCACAGGCTGATGGATCAGCTCGAATG
GCTTCCGGATTCTCTAGGCAAGTTATCGAGTCTTGTCAGGCTAGATCTGTCTGAGAATTG
TATCATGGTACTACCGGCAACCATAGGAGGGCTTATTTCTTTGACGAGGCTTGATTTGCA
TTCGAATAGAATTGGTCAGCTCCCTGAGTCTAT

Last Updated on 06.12.2018 16:11