Sequences from confirmation amplicon


Confirmation sequences for line 065B11 and hit At5g45870 are shown.
Line ID 065B11
Locus At5g45870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18606622
Primers used for PCR 16rr - 8474, show primer details
   
Sequence Name 53-K116102-0022-065-B11C-16rr
GenBank Accession LN508261 [GenBank]
Sequencing primer gene specific primer, 16rr: 5'-AGGAAAACAAAATTGTGAAACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116102-0022-065-B11C-16rr
GAACGCATGTATGCGGTTCGACGTAGTACAAACTATCAATGCTCCGTTACCTCTAGTATG
GTCGATCCTACGTCGATTTGATAACCCAAAAACATTTAAACATTTCGTAAAAACTTGTAA
ACTACGCTCCGGCGATGGAGGAGAAGGCTCTGTCCGTGAAGTCACGGTGGTTTCCGATCT
TCCGGCGAGTTTTAGCCTAGAAAGATTAGATGAACTTGACGATGAGTCTCATGTGATGGT
GATCAGCATTATTGGTGGTGATCATCGTTTGGTTAATTACCAGTCGAAAACTACGGTGTT
TGTGGCGGCGGAGGAGGAGAAGACAGTGGTTGTGGAGAGTTATGTGGTGGATGTGCCGGA
AGGAAATACTGAGGAAGAAACTACGTTGTTTGCTGATACTATCGTTGGTTTTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K116101-0022-065-B11C-8409
GenBank Accession LN501961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116101-0022-065-B11C-8409
TTTAAACCACGATAGTATCAGCAAACAACGTAGTTTCTTCCTCAGTATTTCCTTCCGGCA
CATCCACCACATAACTCTCCACAACCACTGTCTTCTCCTCCTCCGCCGCCACAAACACCG
TAGTTTTCGACTGGTAATTAACCAAACGATGATCACCACCAATAATGCTGATCACCATCA
CATGAGACTCATCGTCAAGTTCATCTAATCTTTCTAGGCTAAAACTCGCCGGAAGATCGG
AAACCACCGTGACTTCACGGACAGAGCCTTCTCCTCCATCGCCGGAGCGTAGTTTACAAG
TTTTTACGAAATGTTTAAATGTTTTTGGGTTATCAAATCGACGTAGGATCGACCATACTA
GAGGTAACGGAGCATTGATAGTTTGTACTACTGTCGAACCGCATACATGCTGTTCTTGAG
ATGTTTTCATACGTGTTTGTTTGGTGTTTCACAATTTGGTTTTCCTAAAAAA

Last Updated on 06.12.2018 16:11