Sequences from confirmation amplicon


Confirmation sequences for line 065E10 and hit At1g13280 are shown.
Line ID 065E10
Locus At1g13280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4548445
Primers used for PCR CW26 - 8474, show primer details
   
Sequence Name 08-K104151-0022-065-E10C-CW26
GenBank Accession LN489707 [GenBank]
Sequencing primer gene specific primer, CW26: 5'-GGAGTGTTGGACTGATACTCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104151-0022-065-E10C-CW26
GTTATGACCAAGTCTATGGATATATATACGCAAACTGAAACAAGTACATAAAATTTAGAA
AAAATTATTCTACTACAATCTTTTTTTTTGTAAGAATAAAAATTGTAAAAGATCAAAACT
ATTCTCGTCTAGCAAAGTCCTTAATTAATGAACAGTTGCAAATAGATTTCTCCTTTATGC
TACACAAAATTGGTTTTCTTCTGAAAAAAAGTGTATACCAGATAAAATTACTATGTTTAA
AACCGATTCAGGGGATGAAAAATACAAAATAAGCATTCGGTGAAAGAACATTAAAAAGAT
TCGTTTATGAGTTTTAGCTCAAACATGGCCACGAGCCTACTAAGCCAAACTTTCCAAATA
TAGAAAGTATCCATGGATTTGATATAATCTATCAAAAAAAAATATCGGAGGGTGGAGTTC
ACGCGCTTAAATCCGGCTAATAGGGACGGGACACATTACGCTAACAAGAACGGCCCATTA
CAAAGGCTCATTATTTAGGCCCATTATTAAAATGTAAACACAATGAACAACAGCCCAATA
AAATCCATGACAAAAACCCCCACAAAGATTTAAATTTCATTTAGTAAAGTTAGCGATCGT
AGCGCCGGGCTGAGTAATGAGTATAATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCATTTCAAAAATGTAAATTTCCGCACGGTTTT
   
Sequence Name 08-K104152-0022-065-E10C-8409
GenBank Accession LN489708 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104152-0022-065-E10C-8409
CGCTACTTACAATTGAATCAAATCTTTGTGGGTGTCTTTGTCATGGATCTTATTGGGCTG
TTGTTCATTGTGTTTACATTTTAATAATGGGCCTAAATAATGAGCCTTTGTATTGGGCCG
TTCTCGTAAGCGTAATGTGTCCCGTCCCTATAAGCTGGATTTAAGCGCGTGAACTCCACC
TTCCGATATTTTTTTTTGATAGATTATATCAAATCCATGGATACTTTCTATATTTGGAAA
GTTTGGCTTAGTAGGCTCGTGGCCATGTTTGAGCTAAAACTCATAAACGAATCTTTTTAA
TGTTCTTTCACCGAAGGCTTATTTTGTATTTTTCATCCCCTGAATCGGTTTTAAACATAG
TAATTTTATCTGGTATACACTTTTTTTCAAAAAAAAACCAATTTTGTGTAGCAAAAAGGA
GAAATCTATTTGCAACTGTTCATTAATTAAGGACTTTGCTAGACAAGAATAGTTTTGATC
TTTTACAATTTTTATTCTTACAAAAAAAAAGATTGTAGTAAAATAATTTTTTCTAAATTT
TATGTACTTGTTTCAGTTTGCGTATATTTTTCCATAGACTTTGGTCATAAACTTATACTT
TAATGAAGTCATGTTGAAGGGAGTATCAT
Primers used for PCR 18wy - 3144, show primer details
   
Sequence Name 63-K118893-0022-065-E10C-18wy
GenBank Accession LN511094 [GenBank]
Sequencing primer gene specific primer, 18wy: 5'-TCGGTTTCTCTAGATCCTTCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118893-0022-065-E10C-18wy
TCTCTCCCCGAGCAGATCCACCACTTCCTCCACTGGAGGTTTCTTCAGAACCATTTGCAG
CAGCAGTAGCAATGACTACTCCAGACCAAGTAAGTAAAAAGTTAACCTTTATGCTCACAA
CTAAGTACCCATTTCATGATTTGAGAAAATTAGCATCAAATTAGGTATAAGAACAAAAAA
AGAATCAACCTTTAAGCTCAAAATCAAGTACCCATTTCACAAAAACTGATTTTTATTTTG
TGAAAAATCTCAGCTAAGATCCAAGAGCTTAACGTCTACGAGTTCAACGAAGGTGATCGA
AACAGTCCAGCGGTTTTAAAACTAGGGAAAAAACCAGATCAGCTTTGTCTCGGAGATCTT
GTCCCCTTCACAAACAAACTCTACACAGGAGATCTCACAAAACGCATAGGAATCACCGCA
GGTCTCTGCGTTTTGATCCAACATGTTCCAGAGAAGAAAGGTGATCGCTTTGAAGCTTCT
TACAGTTTCTACTTCGGAGATTATGGTCACATATCAGTTCAAGGACCTTATCTCACTTAC
GAGGACACGTTTCTTGCTATCACCGGTGGCTCCGGCGTGTTTGAAGGAGCTTATGGACAG
GTCAAGCTTCGTCAGCTTGTGTATCCTACGAAGCTGTTTTACACTTTCTACTTGAAAGGT
GTTGCTGCTGGTTTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAGATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATTCCAC
   
Sequence Name 23-K118892-0022-065-E10C-35St
GenBank Accession LN496773 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118892-0022-065-E10C-35St
TCCACTATCCTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGCGTACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAAACCA
GCAGCAACACCTTTCAAGTAGAAAGTGTAAAACAGCTTCGTAGGATACACAAGCTGACGA
AGCTTGACCTGTCCATAAGCTCCTTCAAACACGCCGGAGCCACCGGTGATAGCAAGAAAC
GTGTCCTCGTAAGTGAGATAAGGTCCTTGAACTGATATGTGACCATAATCTCCGAAGTAG
AAACTGTAAGAAGCTTCAAAGCGATCACCTTTCTTCTCTGGAACATGTTGGATCAAAACG
CAGAGACCTGCGGTGATTCCTATGCGTTTTGTGAGATCTCCTGTGTAGAGTTTGTTTGTG
AAGGGGACAAGATCTCCGAGACAAAGCTGATCTGGTTTTTTCCCTAGTTTTAAAACCGCT
GGACTGTTTCGATCACCTTCGTTGAACTCGTAGACGTTAAGCTCTTGGATCTTAGCTGAG
ATTTTTCACAAAATAAAAATCAGTTTTTGTGAAATGGGTACTTGATTTTGAGCTTAAAGG
TTGATTCTTTTTTTGTTCTTATACCTAATTTGATGCTAATTTTCTCAAATCATGAAATGG
GTACTTAGTTGTGAGCATAAAGGTTAACTTTTTACTTACTTGGTCTGGAGTAGTCATTGC
TACTGCTGCTGCAAATGGTTCTGAAGAAACCTCCAGTGGAGGAAGTGGTGGATCTTGCTC
GTGTGGAGAGACCTGGACTGTTAGATGAGATCCTTTGGTTATGGAAGGATCTAG

Last Updated on 06.12.2018 16:11