Sequences from confirmation amplicon


Confirmation sequences for line 065H10 and hit At3g20190 are shown.
Line ID 065H10
Locus At3g20190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7045876
Primers used for PCR KE27 - 8474, show primer details
   
Sequence Name 37-K100996-0022-065-H10C-8409
GenBank Accession LN502545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K100996-0022-065-H10C-8409
GAGCCATTTAAATTGAATATATCCTGAATCACTCGAACATGGGCTTAGTGGAGGACCACA
CAAGTTCTTGTTCCCTGCAATGTCAACAAAGCAAGAAAGATTCTGTTTTTACATAAGAAC
TCCTATAGTTTACCAAACATTTGCAGATGTACTTGAACTGTTTGTTTAATTACCTGAAAA
GGAGACAGGATCCATGTTGCTAAGGCTTTCCGGTATAGGTCCCTCGAGGTCATTGTTTTC
GAAGCTAGCCAACTTAAGGTCCTTTTGTTTAAAATAAGGTATTTCCCCATGAAACTGATT
CCCATTTAGCCTCAACTCTAAAAGCATTGGCAATAAAGCTAA
   
Sequence Name 37-K101000-0022-065-H10C-KE27
GenBank Accession LN502546 [GenBank]
Sequencing primer gene specific primer, KE27: 5'-TTTAGCTTATTTGCCAATGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K101000-0022-065-H10C-KE27
GATAGTTTCTGGGGAAATCCTTATTTTAACAAAAGGACCTTAAGTTGGCTAGCTTCGAAA
ACAATGACCTCGAGGGACCTATACCGGAAAGCCTTAGCAACATGGATCCTGTCTCCTTTT
CAGGTAATTAAACAAACAGTTCAAGTACATCTGCAAATGTTTGGTAAACTATAGGAGTTC
TTATGTAAAAACAGAATCTTTCTTGCTTTGTTGACATTGCAGGGAACAAGAACTTGTGTG
GTCCTCCACTAAGCCCATGTTCGAGTGATTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11