Sequences from confirmation amplicon


Confirmation sequences for line 066C12 and hit At3g04290 are shown.
Line ID 066C12
Locus At3g04290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1134974
Primers used for PCR 02ew - 3144, show primer details
   
Sequence Name 22-K110678-0022-066-C12C-02ew
GenBank Accession LN496161 [GenBank]
Sequencing primer gene specific primer, 02ew: 5'-GACTGATGCGATCAAATCAACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110678-0022-066-C12C-02ew
TTGGAGGCGCCATAGCACTCGCCATTGCGGCTATGCTGAGCCAGCTCCGCGGGAGCACAT
CCCATTGCACCAGTTCCAGTTACCAAAACTCGTCTTGCACCCAGTTCGTAGAGTTTCTAT
TTCAATGAAGACCAAACAAGAAAACAAATGTAATCATACACCATCAAAGGAGAACTAAGT
ACATCATTAAGTGAACAAAGAAAAAGCTATTAAATTTGCCCCATCATTTTTTGTACGTAC
GTTATGAACTTATGATCACTCTATTCAAGAGAATCATGACATTAACTATGTACGTAGTGA
CATAACGAAAGTAATTATTTGAGAGTTCACATATTAAATGCAATTTCCTTTAGTGAGGCA
ACAAGTTAATAAACTAAATGGTATTGTAGCAAACACTGATAGTTTAAACCGAAGGCGGGA
AACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAANGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TC
   
Sequence Name 06-K110677-0022-066-C12C-3144
GenBank Accession LN488864 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110677-0022-066-C12C-3144
TCCACGATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCT
ACAATACCATTTAGTTTATTAACTTGTTGCCTCACTAAAGGAAATTGCATTTAATATGTG
AACTCTCAAATAATTACTTTCGTTATGTCACTACGTACATAGTTAATGTCATGATTCTCT
TGAATAGAGTGATCATAAGTTCATAACGTACGTACAAAAAATGATGGGGCAAATTTAATA
GCTTTTTCTTTGTTCACTTAATGATGTACTTAGTTCTCCTTTGATGGTGTATGATTACAT
TTGTTTTCTTGTTTGGTCTTCATTGAAATAGAAACTCTACGAACTGGGTGCAAGACGAGT
TTTGGTAACTGGAACTGGTGCAATGGGATGTGCTCCCGCGGAGCTGGCTCAGCATAGCCG
CAATGGCGAGTGCTATGGCGCCCTCCAAACAGCAGCCGCTTTTGTCAAT

Last Updated on 06.12.2018 16:11