Sequences from confirmation amplicon


Confirmation sequences for line 066F04 and hit At1g22990 are shown.
Line ID 066F04
Locus At1g22990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8139560
Primers used for PCR 06op - 8474, show primer details
   
Sequence Name 45-K114782-0022-066-F04C-06op
GenBank Accession LN505625 [GenBank]
Sequencing primer gene specific primer, 06op: 5'-TAAGCATCACTAGCTCTCTCCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114782-0022-066-F04C-06op
ACATGGGAGCTCTCAATTATCTTTCTGAGTATTTCTCTAACCATTTCTACGTCTCAATAC
GAAAACGGAAGAAACGTAAAGTCATGCAGGTAACTAAAATCTCTAAACGCAGCCGTTTAG
TTGTTGTTTCGTCGTTTCTTAGTTGCTTAACCTTTTCAAAATTTTGCTAATAAACAGACG
GTGAATATTAAGGTGAAGATCGACTGTGACGGATGCGAACGCAAAATCAAGAACGCTGTT
TCCTCCATCAAAGGTTCGTTCTTCAACTCTCAAGTTCCACGTAGTCAATTAAATACTACT
CGCCTTTCTATTAGTTAGGGCAAACAATTAATTATGCTTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 45-K114769-0022-066-F04C-8409
GenBank Accession LN505624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114769-0022-066-F04C-8409
CTCCGTGGAAACTTGAGAGTTGAAGAACGAACCTTTTGAGGAGGAAACAGCGTTCTTGAT
TTTGCGTTCGCATCCGTCACAGTCGATCTTCACCTTAATATTCACCGTCTGTTTATTAGC
AAAATTTTGAAAAGGTTAAGCAACTAAGAAACGACGAAACAACAACTAAACGGCTGCGTT
TAGAGATTTTAGTTACCTGCATGACTTTACGTTTCTTCCGTTTTCGTATTGAGACGTAGA
AATGGTTAGAGAAATACTCAGAAAGATAATTGAGAGCTCCCATGTTTACTGTCACAAGCG
AGCAGAGAGATGGAGAGGGAGAGGGAGAGGGAGA

Last Updated on 06.12.2018 16:11