Sequences from confirmation amplicon


Confirmation sequences for line 066H06 and hit At3g49560 are shown.
Line ID 066H06
Locus At3g49560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18371348
Primers used for PCR 04jw - 8474, show primer details
   
Sequence Name 47-K114782-0022-066-H06C-04jw
GenBank Accession LN506280 [GenBank]
Sequencing primer gene specific primer, 04jw: 5'-AAAGAACAGAGAATAGCTCCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114782-0022-066-H06C-04jw
AGTATCAGAGAAGTTGAATTCAACCTCTGGATATGATCTAGTATCATAAGTCTTGGCCCT
GGTGGGATGTTCGCATCTTTCAGCGCGCTGGATTAATAAAGTCATTAACTTTAGTTGCTA
ACATAAACAAAGGCTAGCAGAAACTTTACATGGAGTTCAGATAATTACCTATCAGTGAGC
AATGGCAATGTGGGATCAGTTAAAAGTCCTTTCTTGAAGTTCTTCTCGTATTTCTCCAAA
CCTAGCTTCACTAGCATTGTCCTTCCTCTTGTGAAAAATGGATCTTCAGTACTCGGTTTA
GAGAATCTTTCTCCCAACTAAGGAATCAAACCAAAAAAAATGCTTATATACNGTTTCCAT
GAGTAGAGAGAGTTTTCAATATGATCACTCACTTTAAAAAACACTCCTTGAAAAACAGCA
AAACCAGCAGCAGTAGTGATTGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCANAAATGTAGA
   
Sequence Name 47-K114769-0022-066-H06C-8409
GenBank Accession LN506279 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114769-0022-066-H06C-8409
AAAAACTCCTCTCTACTCATGGAAACCGTATATAAGCATTTTTTTTCGGTTTCGATTCCT
TAGTTGGGAGAAAGATTCTCTAAACCGAGTACTGAAGATCCATTTTTCACAAGAGGAAGG
ACCATTGCTTAGTGAAGCTAGGTTTGGAGAAATACGAGAAGAACTTCAAGAAAGGACTTT
TAACTGATCCCACATTGCCATTGCTCACTGATAGGTAATTATCTGAACTCCATGTAAAGT
TTCTGCTAGCCTTTGTTTATGTTAGCAACTAAAGTTAATGACTTTATTAATCCAGCGCGC
TGAAAGATGCGAACATCCCACCAGGGCCAAGACTTATGATACTAGATCATATCCNGAGGT
TTGAATTCAACTTCTCTGATACTCCATTGATCAAATATACGAATAATTGCTGAATTTGTG
GGAC

Last Updated on 06.12.2018 16:11