Sequences from confirmation amplicon


Confirmation sequences for line 066H12 and hit At2g27040 are shown.
Line ID 066H12
Locus At2g27040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11537748
Primers used for PCR 06ew - 8474, show primer details
   
Sequence Name 25-K110678-0022-066-H12C-06ew
GenBank Accession LN497494 [GenBank]
Sequencing primer gene specific primer, 06ew: 5'-ATTAAAACGATTCAGAGGAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K110678-0022-066-H12C-06ew
TAGTTTGAGTGATAGATGAACTTACATCATTCCAGCGTGAGCACAGAGGTAGAAATCATT
GTTCTTTGGGTGACATATTTTGTTGTCAATGATTGTCCCTGCAAAATAACCAAAACCCCC
ATGAGATAGAGATCAGCTATGTGTCAAAAAAGGTTATAATATAGATTTGTCAATCACCTG
GAGGAACATTTTCAGGAGACGTTGGCTGGAAGAACTTGGTATGATGATTCTTTTGAGCCA
CCAACAAAAGGAACTTTGGGTTCCAATTTGCGTCTAAGAGCTTGCAAGCCTGAGGAAAGA
GAAATGCAATTAAGTACTAACAGAAGAAGAAAATTACACTGAGAAACTTTTATTTTGAAG
TTTCAACTACCTCGATGATCTGATCAAGTTCAATATTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K110677-0022-066-H12C-8409
GenBank Accession LN490232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K110677-0022-066-H12C-8409
GAGCCATTTACAATTGAATATATCCTGAATATTGAACTTGATCAGATCATCGACGTATGT
TGAAACTTCAAAATAAAAGTTTCTCAGTGTAATTTTCTTCTTCTGTTAGTACTTAATTGC
ATTTCTCTTTCCTCAGGCTTGCAAGCTCTTAGACGCAAATTGGAACCCAAAGTTCCTTTT
GTTGGTGGCTCAAAAGAATCATCATACCAAGTTCTTCCAGCCAACGTCTCCTGAAAATGT
TCCTCCAGGTGATTGACAAATCTATATTATAACCTTTTTTGACACATAGCTGATCTCTAT
CTCATGGGGGTTTTGGTTATTTTGCAGGGACAATCATTGACAACAAAATATGTCACCCAA
AGAACAATGATTTCTACCTCTGTGCTCACGCTGGAATGATTGTAAGTTTCATCTATCAAC
TCAAAACTTTATTTGTTAATGTTTGCTCCTCTGAATCGTTTTA

Last Updated on 06.12.2018 16:11