Sequences from confirmation amplicon


Confirmation sequences for line 067D01 and hit At3g01260 are shown.
Line ID 067D01
Locus At3g01260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:80925
Primers used for PCR 02tg - 8474, show primer details
   
Sequence Name 25-K116871w-0022-067-D01C-02tg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02tg: 5'-CGTGTAGCCAGTAAAAAGGAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116871w-0022-067-D01C-02tg
TATGTTTTAATTATTACGTTCTAATTTAAGTGGACTTCATAAATCATAAATGACAAAATT
CATAATTAACATTTTTCTCTAATTAATCAGGTGGGACGAAAGAGTCTAGCGATGTGATTT
GGAGAGTTAAGAAACACAAACATAATGGCAAAAAACCTTATATTGTTTTCACATACACAA
CAAGTCCTGATGGCGATCAAGGTTCGTTTTTTATCCTTAAAAACTTTATCTGTATATCTT
CTTTTTTCTATTATATATGCATAAATGTTAAGATAGAGAAGGTATTCATGTTATTCAATA
GGGAAACTTGAAGTCACTGTAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTATAAAT
   
Sequence Name 05-K116870w-0022-067-D01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116870w-0022-067-D01C-8409
TATATTCTACANTGACTTCAAGTTTCCCTATTGAATAACATGAATACCTTCTCTATCTTA
ACATTTATGCATATATAATAGAAAAAAGAAGATATACAGATAAAGTTTTTAAGGATAAAA
AACGAACCTTGATCGCCATCAGGACTTGTTGTGTATGTGAAAACAATATAAGGTTTTTTG
CCATTATGTTTGTGTTTCTTAACTCTCCAAATCACATCGCTAGACTCTTTCGTCCCACCT
GATTAATTAGAGAAAAATGTTAATTATGAATTTTGTCATTTATGATTTATGAAGTCCACT
TAAATTAGAACGTAATTAATTAAAACATATTTACCATGGATCGTGTTTTTGCCATCGTTT
GCAGCGGCCTTTTCCTTTTTACTGGCTACACG

Last Updated on 06.12.2018 16:11