Sequences from confirmation amplicon


Confirmation sequences for line 068A03 and hit At3g19590 are shown.
Line ID 068A03
Locus At3g19590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6805945
Primers used for PCR IL02 - 8474, show primer details
   
Sequence Name 49-K022563-022-068-A03C-8409
GenBank Accession LN506696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K022563-022-068-A03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGATTTGATTCGAGTCCTCTCTTTCCTTC
ATCTGAAGTTTTTTTTTCTGTTGTTCTGCGTTTGTTGAATCTAAGCGTGTGAGATTGTAT
GATGTGAGCACCAATTCGTTGAAAGGAGAGTTCTTACATGGCGGAGCAGTACTCGATTGC
TGTTTTCACGATGACTTCTCCGGCTTCAGTGTTGGCGCTGATTACAAAGTCCGACGGTAT
TGTCTCTCTTTTCTCTCAGTCATGTGAAGAGACTGTTGGCATAATAATGTTTCCCCTCAG
GCTAATTTAACATTGGCTGCTCAGAAGTTGTTGATTTTACTGCGATTTTTTCTTCATATT
TTGTCACTGTGACGGCTATGGAATCTTTCTACATTTAGCAAATGCCTTGAATCCGTTGTA
TAATTTCCTTAATTTATAAGGTTTCATCTGGTTATACTGCAGGATTGTATTCAATGTCGG
CAAAGAGGACATTTTGGGGACACATGACAAAGCAGTGCGATGT
   
Sequence Name 50-K022563-022-068-A03C-IL02
GenBank Accession LN507040 [GenBank]
Sequencing primer gene specific primer, IL02: 5'-AAGAATACTCAACACATCGCACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K022563-022-068-A03C-IL03
TTTGTCATGTGTCCCCAAAATGTCCTCTTTGCCGACATTGAATACAATCCTGCAGTATAA
CCAGATGAAACCTTATAAATTAAGGAAATTATACAACGGATTCAAGGCATTTGCTAAATG
TAGAAAGATTCCATAGCCGTCACAGTGACAAAATATGAAGAAAAAATCGCAGTAAAATCA
ACAACTTCTGAGCAGCCAATGTTAAATTAGCCTGAGGGGAAACATTATTATGCCAACAGT
CTCTTCACATGACTGAGAGAAAAGAGAGACAATACCGTCGGACTTTGTAATCAGCGCCAA
CACTGAAGCCGGAGAAGTCATCGTGAAAACAGCAATCGAGTACTGCTCCGCCATGTAAGA
ACTCTCCTTTCAACGAATTGGTGCTCACATCATACAATCTCACACGCTTAGATTCAACAA
ACGCAGAACAACAGAAAAAAAAACTTCAGATGAAGGAAAGAGAGGACTCGAATCAAATCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA

Last Updated on 06.12.2018 16:11