Sequences from confirmation amplicon


Confirmation sequences for line 068B10 and hit At1g04120 are shown.
Line ID 068B10
Locus At1g04120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1068373
Primers used for PCR IW52 - 8474, show primer details
   
Sequence Name 45-K033213-022-068-B10C-IW52
GenBank Accession LN505434 [GenBank]
Sequencing primer gene specific primer, IW52: 5'-AGAGTGAGATTGGAAGAAATGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033213-022-068-B10C-IW52
TTCTCAGGCTTTTGTTACTTTTATCTTTTGGAGTTCCCCCATTTTTGTCGCCGCAGTTAC
ATTCGCTACTTCGATATTTCTAGGCACTCAACTTACCGCTGGAGGTGTTCTTTCTGCTCT
GGCGACATTCAGAATTCTTCAGGAGCCACTTCGGAACTTTCCTGATCTGGTTTCAATGAT
GGCTCAGACAAAGGTTTCTCTTGATAGGATTTCTGGGTTCTTGCAGGAGGAAGAACTTCA
AGAAGATGCAACTGTTGTTATTCCACGGGGACTTTCGAATATAGCCATAGAGATTAAAGA
TGGTGTGTTTTGTTGGGACCCTTTTTCTTCAAGGCCGACATTATCTGGGATTCAGATGAA
AGTGGAGAAGGGTATGCGTGTGGCTGTCTGTGGCACAGTTGGCTCTGGAAAATCAAGTTT
TATCTCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCACTCCCAAAATGG
   
Sequence Name 37-K033213-022-068-B10C-8409
GenBank Accession LN502519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K033213-022-068-B10C-8409
GACCATTTACTGGAGATAAAACTTGATTTTCCAGAGCCAACTGTGCCACAGACAGCCACA
CGCATACCCTTCTCCACTTTCATCTGAATCCCAGATAATGTCGGCCTTGAAGAAAAAGGG
TCCCAACAAAACACACCATCTTTAATCTCTATGGCTATATTCGAAAGTCCCCGTGGAATA
ACAACAGTTGCATCTTCTTGAAGTTCTTCCTCCTGCAAGAACCCAGAAATCCTATCAAGA
GAAACCTTTGTCTGAGCCATCATTGAAACCAGATCAGGAAAGTTCCGAAGTGGCTCCTGA
AGAATTCTGAATGTCGCCAGAGCAGAAAGAACACCTCCAGCGGTAAGTTGAGTGCCTAGA
AATATCGAAGTAGCGAATGTAACTGCGGCGACAAAAATGGGGGAACTCCAAAAGATAAAA
GTAACAAAAGCCTGAGAATATAAGGCTTTGCGAAGCCAACCATACTCCTCTTCCCTCATT
TCTTCCAANCTCACT
Primers used for PCR 55xb - 3144, show primer details
   
Sequence Name 71-K118874-0022-068-B10C-55xb
GenBank Accession LN512640 [GenBank]
Sequencing primer gene specific primer, 55xb: 5'-CTTCAGAACCTTTGAAACAACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118874-0022-068-B10C-55xb
CGGAACAGTTTGGAACTCCTTCAACAATTTTACCTTTCTGCAACAATAGAGATAAGTTCA
TCACAGGGCTGGTAAGCTAAACACCTAAAAGAGACCCTTTAGCAATTCTAAAAGGACCAA
CAGTAGCCAGAATTAATGTGTAGTAAAACCGAGAAAAAAGAGTTTCTTACCAATATTAGA
TCAGCTGCAGGGAGAAACTCAACTTGATGCGTTACAAAAACCACAGTTTTCTCAGCCAAT
GCAGATAGAATATAATCCTGTATAGCCATTAATAACCGTGTTACGCAAACGTTCAAACTC
AAAATATGAAAACATGACTTGAATAGCTTCTTACCCTAAACAAATCAGAGCCAGTGTGTG
CATCAAGAGCACTAAAAGGGTCGTCTAGTAAATAAATGTCAGCATCTTGATATAATGCCC
TTGCAAGTTGTACACGCTGTTTCTGACCTCCGCTGAGATTTATACCTCTCTCCCCGATAA
TAGTTTGGTCCCCATGTGAGAAAAGCTCTATATCTTTCTTTAGGGAACATGCTTGTATCA
CATTCTTGTACTTTGTTTTCTCCATTGGACTGCCAAATAGAATGTTTTCTTCAATGTTAC
CAGACTGAATCCAAGCCGATTGAGACACATAACCAGTAGTACCACATATTCTAACCTATA
TAACAATTATCAGCAGTTGGATCAATGATAGCTAAGTGAATATNAGAAAAGAAAAGAAAA
AATAGTGAAGCAATTGAGACAAACACCCCAAAACATTAACTTACTTCGCCAGAGATTTTT
GATAAATCTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAG
   
Sequence Name 31-K118873-0022-068-B10C-3144
GenBank Accession LN500356 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118873-0022-068-B10C-3144
ACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGG
GTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATTTATCAAAA
ATCTCTGGCGAAGTAAGTTAATGTTTTGGGGTGTTTGTCTCAATTGCTTCACTATTTTTT
CTTTTCTTTTCTTATATTCACTTAGCTATCATTGATCCAACTGCTGATAATTGTTATATA
GGTTAGAATATGTGGTACTACTGGTTATGTGTCTCAATCGGCTTGGATTCAGTCTGGTAA
CATTGAAGAAAACATTCTATTTGGCAGTCCAATGGAGAAAACAAAGTACAAGAATGTGAT
ACAAGCATGTTCCCTAAAGAAAGATATAGAGCTTTTCTCACATGGGGACCAAACTATTAT
CGGGGAGAGAGGTATAAATCTCAGCGGAGGTCAGAAACAGCGTGTACAACTTGCAAGGGC
ATTATATCAAGATGCTGACATTTATTTACTAGACGACCCTTTTAGTGCTCTTGATGCACA
CACTGGCTCTGATTTGTTTAGGGTAAGAAGCTATTCAAGTCATGTTTTCATATTTTGAGT
TTGAACGTTTGCGTAACACGGTTATTAATGGCTATACAGGATTATATTCTATCTGCATTG
GCTGAGAAAACTGTGGTTTTTGTAACGCATCAAGTTGAGTTTCTCCCTGCAGCTGATCTA
ATATTGGTAAGAAACTCTTTTTTCTCGGTTTTACTACACATTAATTCTGGCTACTGTTGG
TCCTTTTAGAATTGCTAAAGGGTCTCTTTTAGGTGTTTAGCTTACCAGCCTGTGATGAAC
TTATCTCTATTGTTGCAGAAAGGTAAAATTGTTGAAGGAGTTTCAAACTGTTTCCGTTAT
GATAAGCTCTGAAATATTTATTTGTG

Last Updated on 06.12.2018 16:11