Sequences from confirmation amplicon


Confirmation sequences for line 069C05 and hit At5g09950 are shown.
Line ID 069C05
Locus At5g09950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3103019
Primers used for PCR mr34 - 8474, show primer details
   
Sequence Name 25-K019224-022-069-C05C-8409
GenBank Accession LN497236 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K019224-022-069-C05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATAACCTCAGAGTTTGCGGTGATTGC
CACTCTGCCTTCAAGTATATATCAAAGATCGAAGGGAGACAGATAATTCTAAGGGATTCA
AACAGGTTTCATCATTTTCAAGACGGTGCATGTTCTTGTAGTGATTTCTGGTGATACCAT
GAGTCTCAAAGGTATGAAGACCATCAACCTCCAATCGAGATTCCTTTTAAATCTCTTATT
CATGTATGCTCTGTTTCTTCAGATGATTTTACTTGGATTCAGCATTCATTGACTTAAACC
ATAGTTCTTTCCCATATGGAATGCGAAGTTCAGATGCATCAAATCAGTGAGTTACAATTT
TTATCCTTTATATATTAACCTTAAACTTTGTCTGGCAAGTTAAGAATCTATTTTCTATTT
TCGAAAATATGTGTGAAAAAGCTCAAGGCT
   
Sequence Name 29-K019224-022-069-C05C-mr34
GenBank Accession LN499091 [GenBank]
Sequencing primer gene specific primer, mr34: 5'-ATGTCATATGAGCCTTGAGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K019224-022-069-C05C-MR34
CACATATTTTCGAAAATAGAAAATAGATTCTTAACTTGCCAGACAAAGTTTAAGGTTAAT
ATATAAAGGATAAAAATTGTAACTCACTGATTTGATGCATCTGAACTTCGCATTCCATAT
GGGAAAGAACTATGGTTTAAGTCAATGAATGCTGAATCCAAGTAAAATCATCTGAAGAAA
CAGAGCATACATGAATAAGAGATTTAAAAGGAATCTCGATTGGAGGTTGATGGTCTTCAT
ACCTTTGAGACTCATGGTATCACCAGAAATCACTACAAGAACATGCACCGTCTTGAAAAT
GATGAAACCTGTTTGAATCCCTTAGAATTATCTGTCTCCCTTCGATCTTTGATATATACT
TGAAGGCAGAGTGGCAATCACCGCAAACTCTGAGGTTATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11