Sequences from confirmation amplicon


Confirmation sequences for line 069H10 and hit At3g21280 are shown.
Line ID 069H10
Locus At3g21280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7479016
Primers used for PCR 07de - 8474, show primer details
   
Sequence Name 46-K109414-0022-069-H10C-07de
GenBank Accession LN505888 [GenBank]
Sequencing primer gene specific primer, 07de: 5'-ACCATTGATTATCGCTTTATTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K109414-0022-069-H10C-07de
TCACATTGGGAAACTTTGAACATTACATATAACCAAATTTTGAATCCAACATATAGACCA
ATTACAGTGCGTTCCAAGCTAGACAAGAAAGATGTTGAGAACCGGTTCTTAGAGAATATA
CCTTCCTGTTGGTCACCAGTTGATGATTCGCCACTTTGGTTTGATGATCCCTGGAGAGAG
ATATTGAGTAAGGATCTTAGAATATTGTATTGAGCGCAGTGTTAATAAGAACACATATTT
TGTACCTCAGCATCAGTCATTTTCACATCATCACCCTTTGAGCTGCTCTTTGCACTGGCT
TGCAGACCAAGCTTTTGACCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 30-K109413-0022-069-H10C-8409
GenBank Accession LN499758 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109413-0022-069-H10C-8409
GCCATTTACAATTGAAGGTCAAAAGCTTGGTCTGCAAGCCAGTGCAAAGAGCAGCTCAAA
GGGTGATGATGTGAAAATGACTGATGCTGAGGTACAAAATATGTGTTCTTATTAACACTG
CGCTCAATACAATATTCTAAGATCCTTACTCAATATCTCTCTCCAGGGATCATCAAACCA
AAGTGGCGAATCATCAACTGGTGACCAACAGGAAGGTATATTCTCTAAGAACCGGTTCTC
AACATCTTTCTTGTCTAGCTTGGAACGCACTGTAATTGGTCTATATGTTGGATTCAAAAT
TTGGTTATATGTAATGTTCAAAGTTTTCCCAATGTGAGTATTTAGAAAAATAGTAGAGAG
ATGGCTACTTGCAAATAAAGCGATAATCAATGG

Last Updated on 06.12.2018 16:11