Sequences from confirmation amplicon


Confirmation sequences for line 070F02 and hit At3g46370 are shown.
Line ID 070F02
Locus At3g46370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17052380
Primers used for PCR KE24 - 8474, show primer details
   
Sequence Name 04-K103490-0022-070-F02C-KE24
GenBank Accession LN487882 [GenBank]
Sequencing primer gene specific primer, KE24: 5'-CAGACAACGTTGAGATACTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103490-0022-070-F02C-KE24
TCTTAGTGTGCATAAAGAAACAAAGTATTTGATTAGGGTTACAAGTAACTATGGAAATTA
CGATGGTCGTAACGAACCTCCTAGATTTGATTTGTACTTAGGCCCTAACTTCTGGGTAAC
AATAGATTTGGGGAAGCATGTAAATGGTGATACATGGAAGGAGATTATTCATATCCCAAA
GTCAAACTCTCTAGATGTGTGTCTTATCAAAACAGGTACAACAACGCCAATTATATCGAC
CTTGGAACTAAGGTCTCTACCAAAGTATAGTTACAACGCAATATCAGGTTCTTTGAAGTC
GACACTGAGGGCATTCCTAAGCGAATCAACAGAAGTTATAAGGTACGTCACCATTAAATT
GATATTAAGGTTTTTTGACTCGTTCCATCGTCCATTGTTAATAATACAAATGAAAATGGC
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K103491-0022-070-F02C-8409
GenBank Accession LN498806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103491-0022-070-F02C-8409
ATTTTCTTTGTATTATTAACAATGGACGATGGAACGAGTCAAAAAACCTTAATATCAATT
TAATGGTGACGTACCTTATAACTTCTGTTGATTCGCTTAGGAATGCCCTCAGTGTCGACT
TCAAAGAACCTGATATTGCGTTGTAACTATACTTTGGTAGAGACCTTAGTTCCAAGGTCG
ATATAATTGGCGTTGTTGTACCTGTTTTGATAAGACACACATCTAGAGAGTTTGACTTTG
GGATATGAATAATCTCCTTCCATGTATCACCATTTACATGCTTCCCCAAATCTATTGTTA
CCCAGAAGTTAGGGCCTAAGTACAAATCAAATCTAGGAGGTTCGTTACGACCATCGTAAT
TTCCATAGTTACTTGTAACCCTAATCAAATACTTTGTTTCTTTATGCACACTAAGATTGT
AACAGTTTCGCATTCCGTCTGGAAAGTATCTCACGGTTGTCTG

Last Updated on 06.12.2018 16:11