Sequences from confirmation amplicon


Confirmation sequences for line 070H09 and hit At3g42850 are shown.
Line ID 070H09
Locus At3g42850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14941238
Primers used for PCR 02gg - 3144, show primer details
   
Sequence Name 30-K111953-0022-070-H09C-02gg
GenBank Accession LN499781 [GenBank]
Sequencing primer gene specific primer, 02gg: 5'-CGTATGTACCATCTTTGTCAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111953-0022-070-H09C-02gg
ATTTTCGAGGATTCTTCTCCTGTGATAGACTGTGGGAGCTTACTTGCATAAAGAGCTTGG
AATCTGCGAAAAAAAAGAAGGAAAAAATATTGATCTCATCTAATTTGATTAACACAGATG
ATATATACATAGAGAGAGATTTATAGTACCTATGAGGTGAAAGGTTACATAAGTAATCCA
ACGAAGTATCGGATTCAATCAACTCCGAGCTCTCCTCTTCAGCCTCTTCTGAGTTGGTTT
CAGCAAAAGAAGCTGCAAAAGATCTTATCATTGTCTTACCAATAAAAGCTCCTATCCTCA
CTGAACCATAGTCTGAACCACCAACGCTGCAGAGTACCAAATTTGTTAGAAACAATAGTT
TTGAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 14-K111952-0022-070-H09C-3144
GenBank Accession LN492569 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K111952-0022-070-H09C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTCAAAACTATTGTTTCT
AACAAATTTGGTACTCTGCAGCGTTGGTGGTTCAGACTATGGTTCAGTGAGGATAGGAGC
TTTTATTGGTAAGACAATGATAAGATCTTTTGCAGCTTCTTTTGCTGAAACCAACTCAGA
AGAGGCTGAAGAGGAGAGCTCGGAGTTGATTGAATCCGATACTTCGTTGGATTACTTATG
TAACCTTTCACCTCATAGGTACTATAAATCTCTCTCTATGTATATATCATCTGTGTTAAT
CAAATTAGATGAGATCAATATTTTTTCCTTCTTTTTTTTCGCAGATTCCAAGCTCTTTAT
GCAAGTAAGCTCCCACAGTCTATCACAGGAGAAGAATTCCTCGAAAAATATGGTGATCAC
GGGGATTCTGTCACAACCATTGACAAAGATGGTACATACG

Last Updated on 06.12.2018 16:11