Genotyping and primer details


Genotyping and primer details for 070H09 - At3g42850
Line ID 070H09
Locus At3g42850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:14941238 of line 070H09 Chr3:14941238
   
Insertion allele amplicon 3144 - 02gg
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 02gg: 5'-CGTATGTACCATCTTTGTCAATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:14940831
   
wt amplicon (over InsPos) 01ob - 02gg
wt amplicon start Chr3:14940831
wt amplicon end Chr3:14941727
wt amplicon size 897 bp
Locus specific primer 02gg: 5'-CGTATGTACCATCTTTGTCAATGG-3'
2nd locus specific primer 01ob: 5'-TTTCAGGTTTCTTCAACAGTTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11