Sequences from confirmation amplicon


Confirmation sequences for line 071E01 and hit At2g31710 are shown.
Line ID 071E01
Locus At2g31710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13482433
Primers used for PCR CD34 - 8474, show primer details
   
Sequence Name 12-K104193-0022-071-E01C-CD34
GenBank Accession LN491554 [GenBank]
Sequencing primer gene specific primer, CD34: 5'-TGTGAGAAAAACAGATCAAATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104193-0022-071-E01C-CD34
TTATGGACCGCTGTTATGGTTTTTGTGAATTTTGTCCTACAACATTAGCTATCTTCTCTG
TCCAACATGAACATGTCAAAGTTTTTCTGCTCAACTAACCCAAAACTCACAACACAGAAT
GATCTAGCAACCAAACAATACATTGCAAGAACCTGAGCTCTGCTCCTTCTGATGCAATCT
CGACACTGTTCCTTGTTTAACAAAAATGGGATGTGGCAAGCTCTCACTCTTGTTTCTTCA
GCGCAGGGTCAGTATTTGTGACTCCTGATGTCAATTTCTTAACACTGTCTTTAGCCTCTG
CAACAAACGAAGCGTCTGGCTTGTGTTTATCCGCAGGTTCTTTCAGGGCCAGACAGATGT
AAAACACAATAACTACATTGACTGATACCACGGCAAGAAATCCACTCAGTAGTGTCAGAG
AATGTGGAGACAACGTTGTTGACCCTGTCGAGATTTAACAGAGCAACACGGTCAATTTCT
ACAGCATTTACTGTTAGAAACTAAAGCTGGGAAACTACAACAGACAAGCTACGTGAGCCT
GAGAGAGAAAGAGAGACATTATGCCAAAAGTACAGTAACCGACTAACAGGCTATCACATG
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K104194-0022-071-E01C-8409
GenBank Accession LN498821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104194-0022-071-E01C-8409
GAGCCATTTACCATGTGATAGCCTGTTAGTCGGTTACTGTACTTTTGGCATAATGTCTCT
CTTTCTCTCTCAGGCTCACGTAGCTTGTCTGTTGTAGTTTCCCAGCTTTAGTTTCTAACA
GTAAATGCTGTAGAAATTGACCGTGTTGCTCTGTTAAATCTCGACAGGGTCAACAACGTT
GTCTCCACATTCTCTGACACTACTGAGTGGATTTCTTGCCGTGGTATCAGTCAATGTAGT
TATTGTGTTTTACATCTGTCTGGCCCTGAAAGAACCTGCGGATAAACACAAGCCAGACGC
TTCGTTTGTTGCAGAGGCTAAAGACAGTGTTAAGAAATTGACATCAGGAGTCACAAATAC
TGACCCTGCGCTGAAGAAACAAGAGTGAGAGCTTGCCACATCCCATTTTTGTTAAACAAG
GAACAGTGTCGAGATTGCATCAGAAGGAGCAGAGCTCAGGTTCTTGCAATGTATTGTTTG
GTTGCTAGATCATTCTGTGTTGTGAGTTTTGGGTTAGTTGAGCAGAAAAACTTTGACATG
TTCATGTTGGACAGAGAAGATAGCTAATGTTGTAGGACAAAATTTCACAAAAACCATAAA
CAGCTGTGTCCATAATTGTTTAATTTTAAGCAATTTGATCTGTTTTTCTCAC

Last Updated on 06.12.2018 16:11