Sequences from confirmation amplicon


Confirmation sequences for line 071G03 and hit At4g36070 are shown.
Line ID 071G03
Locus At4g36070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17058286
Primers used for PCR DE14 - 8474, show primer details
   
Sequence Name 19-K019240-022-071-G03C-8409
GenBank Accession LN494562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K019240-022-071-G03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATTTCACTCCTGC
CATTTTCACAAAATCACCTCCATTATCTACCATTCTGCTCTCAGGGACAAGACTTGACAA
GTGAAACTTTTACCTGGCTTTATGAAGTCCGACAGACCAAAATCTGTAGCCTTTAGAGAT
GAACCTTCTTCGGTTGATTTGAACAGAAAATTCTGAGAGGGTAAGGGATCATAACTCTTC
TTAAAGTGATGAAGATGCTTTCACATATGGATTTGCAATTTCCAAGCATTGAATCAAACT
GAATCGTACCTACCTCTGGCTTCATGTCGCGGTGAACTAGACCTCGTAAATGACATTCAG
CTGCAACTTTCAGCATTTGTCTCACCACAACCGCTGCATCTTTCTCGGTGTAACGGCTAT
CTTTCCTGGAAAATATATACACATAAGTTGACTTGATGTAACTTCCCATCTTTTCTTACA
GAATTTTAGCATTACTTCTATGTTAATACAAAGTTTTGTTAATCTTACTTAGCTAGTATT
CGATCTAGCAATTCACCACCGTCACATAACCTGGGAAAAGAAAAACTCATCAAAACCAGA
GTAAATTCATTTTTCTTGTATCACAAGAAAAAGAATGATAGTAAAAACAGAGACAAACTT
GAGACTTACTCCATGACAATGTAAATATAGGTCTTGTCCTCAAATGCATTGTGAAAGCCT
ACCACATTCTCATGCCCACCTAAAGCTTGTAGTATCTTAACCTCTCGCTTCACATCTTCC
ACTTCAATCGGNTGAGTCATCTGTACCACACCAAAA
   
Sequence Name 20-K019240-022-071-G03C-DE14
GenBank Accession LN495018 [GenBank]
Sequencing primer gene specific primer, DE14: 5'-TTAGAGTGTCTCCCAAATCGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K019240-022-071-G03C-DE14
CTTAGCGCGAAAGCGGTACGAGTAACACCTAACTCCATGGCGTTTTTGGTGTGGTACAGA
TGACTCAACCGATTGAAGTGGAAGATGTGAAGCGAGAGGTTAAGATACTACAAGCTTTAG
GTGGGCATGAGAATGTGGTAGGCTTTCACAATGCATTTGAGGACAAGACCTATATTTACA
TTGTCATGGAGTAAGTCTCAAGTTTGTCTCTGTTTTTACTATCATTCTTTTTCTTGTGAT
ACAAGAAAAATGAATTTACTCTGGTTTTGATGAGTTTTTCTTTTCCCAGGTTATGTGACG
GTGGTGAATTGCTAGATCGAATACTAGCTAAGTAAGATTAACAAAACTTTGTATTAACAT
AGAAGTAATGCTAAAATTCTGTAAGAAAAGATGGGAAGTTACATCAAGTCAACTTATGTG
TATATATTTTCCAGGAAAGATAGCCGTTACACCAAGAAAGATGCAGCGGTTGTGGTGAGA
CAAATGCTGAAAGTTGCACCTGAATGTCATTTACGAGGTC

Last Updated on 06.12.2018 16:11