Sequences from confirmation amplicon


Confirmation sequences for line 072C07 and hit At1g76460 are shown.
Line ID 072C07
Locus At1g76460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28687307
Primers used for PCR 20by - 8474, show primer details
   
Sequence Name 91-K108704-0022-072-C07C-20by
GenBank Accession LN515125 [GenBank]
Sequencing primer gene specific primer, 20by: 5'-CTACCAGCAAGGAGTTGTCTATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K108704-0022-072-C07C-20by
TACACACTGATGTCTTTGCGCAAGTGTCTAATGTTTGTTTTTATTTTCTATCTCAGAGTA
ACCGCTTATGGACCCGAGTACATGTACTCGCAATCACAGGTTGACAATGGCTATGAACAC
TTGCTATTATTTCTCTTCGTCATTAAACTCTCTTCTAACTTTGGCTATGTCTTTTCTTGT
TTCATGGTCTAGGGTTTATATAGTCCATACATGGGTCAGCAATACCTTCAGGTCTATGGA
GTACCTGGAGCAGTGAATTCACCCGTTTATCAATACGGGCAACTAAGTCAGACTATTCCT
AATGGCCATGGTTATACAGCAGTTCAAGGCTATTCGGTTCCAGGAAGCCATATTCTGCAG
CTTGGTGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 07-K108703-0022-072-C07C-8409
GenBank Accession LN489309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108703-0022-072-C07C-8409
ATTTCATTGAATATTTCCACCAAGCTGCAGAATATGGCTTCCTGGAACCGAATAGCCTTG
AACTGCTGTATAACCATGGCCATTAGGAATAGTCTGACTTAGTTGCCCGTATTGATAAAC
GGGTGAATTCACTGCTCCAGGTACTCCATAGACCTGAAGGTATTGCTGACCCATGTATGG
ACTATATAAACCCTAGACCATGAAACAAGAAAAGACATAGCCAAAGTTAGAAGAGAGTTT
AATGACGAAGAGAAATAATAGCAAGTGTTCATAGCCATTGTCAACCTGTGATTGCGAGTA
CATGTACTCGGGTCCATAAGCGGTTACTCTGAGATAGAAAATAAAAACAAACATTAGACA
CTTGCGCAAAGACATCAGTGTGTACTGTGTGATATAAGAATTGCAAGACTTTACCCATAA
GGATAGACAACTCCTGNCTGGTAG

Last Updated on 06.12.2018 16:11