Sequences from confirmation amplicon


Confirmation sequences for line 074A11 and hit At2g40960 are shown.
Line ID 074A11
Locus At2g40960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17096791
Primers used for PCR 05sf - 8474, show primer details
   
Sequence Name 27-K116518-0022-074-A11C-05sf
GenBank Accession LN498555 [GenBank]
Sequencing primer gene specific primer, 05sf: 5'-ATCCCTGTGAAACAGTCAGAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116518-0022-074-A11C-05sf
GTGTTTAAGCCAGACCTACTAAAGGATCTGGAATGGAAGGTGATGAACTGAAAGGTGGTC
TTCTGAAAAGTGTTGAAGAGAGGAAAGAAGATTACGACAGGGCTCGAGCACGCATATTTA
ACGGTCTTGTGAACATTGATTGTAACGATTCTTCATCTGAAACTAATCCTTGGAGGGTGA
ATTGTAGTTCTAGCAGAGATGAAAATCAAGTGTTGAAGAATGGTAATATCGAAGCAGACA
AGAACAATATCTCTAGGAAGAGCAATCCTGCATCTCGTATTGCAATTCTCAGAGACAGAG
AGAAAGATCGATATGACCCTGACTATGACCGCAGCAATTACAGGTATGTAAAGCATTGCT
TCATGCTATTCATTTACAGGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 01-K116517-0022-074-A11C-8409
GenBank Accession LN486733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116517-0022-074-A11C-8409
TTGAATAACCTGTATATGAATAGCATGAAGCAATGCTTTCAGTCATGTAATTGCTGCGGT
CATAGTCAGGGTCATATCGACCTTTCTCTCTGTCTCTGAGAATTGCAATACGAGATGCAG
GATTGCTCTTCCTAGAGATATTGTTCTTGTCTGCTTCGATATTACCATTCTTCAACACTT
GATTTTCATCTCTGCTAGAACTACAATTCACCCTCCAAGGATTAGTTTCAGATGAAGAAT
CGTTACAATCAATGTTCACAAGACCGTTAAATATGCGTGCTCGAGCCCTGTCGTAATCTT
CTTTCCTCTCTTCAACACTTTTCAGAAGACCACCTTTCAGTTCATCACCTTCCATTCCAG
ATCCTTTAGTAGGTCTTGGCTTTATAACAACTTTCATATGCTCAAACTTGCCAGTTTCTG
ACTGTTTCACAGGGATA

Last Updated on 06.12.2018 16:11