Sequences from confirmation amplicon


Confirmation sequences for line 076A02 and hit At3g16860 are shown.
Line ID 076A02
Locus At3g16860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5761944
Primers used for PCR ER44 - 8474, show primer details
   
Sequence Name 71-K037325-022-076-A02C-ER44
GenBank Accession LN512507 [GenBank]
Sequencing primer gene specific primer, ER44: 5'-ATTTTGATACCTTGTTGAGTGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K037325-022-076-A02C-ER44
TTACAGAGTGATGTTCTTCGGAAGCGGAACACTCGGCGGAGCAACTCCAAACTGAGTACC
CACGAGCTCAACACGTGCTTCCATCTGAGTTACGTCGCCGGCCGTCATGATCGCCGTCTT
CAAATCCGCCGATGGAAACCCGGCGAAGATTGTCCCGTTCTCAACGCTAGCCGGGAGGCT
AGAACCGTCAGAGAGAACAGCGTTGGTCGCTGAAACAAGAATCTCGCGGTGGGCGAATTT
AACAAAGACTTGCCACGATTTAAGCTCGTCGCGGCCGTTGTTTAGGACCGTGATCTCTGA
TTCGAACCGGTACGGCTGGTTCTTCGTGTCGTTTGGTTTAATCTTGGTACCGGTTAAGTA
AGTGGTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 79-K037325-022-076-A02C-8409
GenBank Accession LN513781 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K037325-022-076-A02C-8409
TACGGTACAGATTAACCAAACGACACGAAGAACCAGCCGTACCGGTTCGAATCAGAGATC
ACGGTCCTAAACAACGGCCGCGACGAGCTTAAATCGTGGCAAGTCTTTGTTAAATTCGCC
CACCGCGAGATTCTTGTTTCAGCGACCAACGCTGTTCTCTCTGACGGTTCTAGCCTCCCG
GCTAGCGTTGAGAACGGGACAATCTTCGCCGGGTTTCCATCGGCGGATTTGAAGACGGCG
ATCATGACGGCCGGCGACGTAACTCAGATGGAAGCACGTGTTGAGCTCGTGGGTACTCAG
TTTGGAGTTGCTCCGCCGAGTGTTCCGCTTCCGAAGAACATCACTCTTGTTAATGATGGA
TGGTCTTGTCCTAAACCCACTCAACAAGTAATCAAAA

Last Updated on 06.12.2018 16:11