Sequences from confirmation amplicon


Confirmation sequences for line 076B01 and hit K18I23 are shown.
Line ID 076B01
Locus K18I23 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1574681
Primers used for PCR EV78 - 8474, show primer details
   
Sequence Name 08-K037321-022-076-B01C-EV78
GenBank Accession LN489651 [GenBank]
Sequencing primer gene specific primer, EV78: 5'-CAAGTTCATGTCCTCTTTCCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K037321-022-076-B01C-EV88
TTTCACTGTGGTAAGACTTAGCCATGAATGATTCTCAATGAGCAAGCACAATGTTAGAGC
ACTGGAGCTTAGTCTAGTGTATTGGTATTTGGCAAATGCAATTAAGAAAATCAAAACCAA
AACTAACGTGAGTTACGTCACCCAATGCATTCGCTTGAATCCCAAGGTACAAATAAAAAG
AAATGTAAAATATGCATAATCTCGTTGCAGGTACAATTTATCATGTTAATTCACACAATA
AACACTCTGCTTAAACGAAATTAAAAAAAAAAAAAAAACTTTCTTCTAAATCAAAATCTG
GAAAAATTGATTCGGGGTCCCCCCTTTGGCCCCGGTAAAGAAAAGGGGACAAATAATGAG
TAATTAGGGTTTCGGGCTTCAACTGTTCAACAAAAAACCCATCTAAAATTAACCCCATTT
CATTATTTTAAAGTGCCCCCAATTTACAACAGGATATATTCAATTGAAAATGGCTTCTTG
TCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAAA
GTAATTCCCATTTTTTTTTCAATTCAAAAATGTAAATGTCCCCAGCGTTTTTAAAAAATA
AAAGTACTTTTTGAAAAAACAACAAATTACAATCCGTCTTATTTATAGGCAAAAGCAAAA
AAAAAATTATTCTAATTCGAAAATTTTTATTTCAACGTGTCTACTTTCCC
   
Sequence Name 16-K037321-022-076-B01C-8409
GenBank Accession LN493352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K037321-022-076-B01C-8409
CCGGACTGAGCCATTTCAATTGAATATATCCTGTTGTAAATTGGGGACACTTTAATATAA
TGAAATGGGCTTAATCTTAGATGGGTTTACTGTTGAACAGTTGAAGCCAGAAACCCTAAT
TACTCATTACTCGTCCACTTTACTTTACCGGCGCCAATGGTGAGACCCTGAATCAATCTT
TCCAGATCTTGATTTAGATGAATGTTTTTTTTTTTTTTTTAATTTCTTTTAACCAAAGGG
TTTTTTGGGGGAATTAACATGAAAAATTGTCCCTGCAACGAGATTATGCATATTTTACTT
TTTTTTTTTTTTGTCCCTGGGGATTCAAGCAAAGGCTTTGGGGGACGTAACTCACGTTAG
TTTTGGTTTTGATTTTCTTAATTGCTTTTGCCAAAAACCAATACCCTAAACTAAGCCCCG
GGGCTCTAACATTGGGCTTGCTCATTGAAAATCATTCAGGGCTAATTCTTCCACAGTTAA
AATGAAACTTTTTCCCCCCCACCCAAAAAGGGAAAAA

Last Updated on 06.12.2018 16:11