Sequences from confirmation amplicon


Confirmation sequences for line 076C04 and hit At5g57010 are shown.
Line ID 076C04
Locus At5g57010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23068682
Primers used for PCR 01ld - 8474, show primer details
   
Sequence Name 55-K113845-0022-076-C04C-01ld
GenBank Accession LN508874 [GenBank]
Sequencing primer gene specific primer, 01ld: 5'-AACTTACGGCTTCTAACCAATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K113845-0022-076-C04C-01ld
AATCCTTTCCACCTATCAGCATATCAAACTATTTTATTAACCAAAGTAAGAATTAATGAT
GATAATGTATGACTAGTGAATAATTAATTATTACCTTTGCTGCTTTTGTTCCAGCTCTTG
CCCACCTTGATACAGCAGACTCTGGTTTATCATTGGTTTTGTTCGGTTCTAACTTTGCCA
ATTCCAATTCTTTCCACCTAAACCAAACACACCACTTAAGTATAATTTTCATATCAACAT
TGGGTTAAAATGTATAAAACTGAAATTCAAACGTACCATAATTCTTTAATTGAATATTCT
TTATGGTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCG
   
Sequence Name 28-K113844-0022-076-C04C-8409
GenBank Accession LN498932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K113844-0022-076-C04C-8409
TACATTGATATATCCTGACACCATAAAGAATATTCAATTAAAGAATTATGGTACGTTTGA
ATTTCAGTTTTATACATTTTAACCCAATGTTGATATGAAAATTATACTTAAGTGGTGTGT
TTGGTTTAGGTGGAAAGAATTGGAATTGGCAAAGTTAGAACCGAACAAAACCAATGATAA
ACCAGAGTCTGCTGTATCAAGGTGGGCAAGAGCTGGAACAAAAGCAGCAAAGGTAATAAT
TAATTATTCACTAGTCATACATTATCATCATTAATTCTTACTTTGGTTAATAAAATAGTT
TGATATGCTTGATAGGTGGGGAAAGGATTGTTGAAAGATGATAAAGCACAAAAATTAGCA
TTGCGACATTGGTTA

Last Updated on 06.12.2018 16:11