Sequences from confirmation amplicon


Confirmation sequences for line 076C06 and hit At5g05540 are shown.
Line ID 076C06
Locus At5g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1638202
Primers used for PCR 05wj - 8474, show primer details
   
Sequence Name 77-K118544-0022-076-C06C-05wj
GenBank Accession LN513609 [GenBank]
Sequencing primer gene specific primer, 05wj: 5'-TGGATCATGCCAGAGTAATTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118544-0022-076-C06C-05wj
CCTTTAGAACTTAAAAGACCTTCTTTGAATTATCTGTGCAAGGTAAAGCCTGTGAAAGCT
CAACCCTCTGCTCATGAGATATGGAATCTCGTATGTACATGACCGATGTATCACATTGTT
TATTTGATTTGATTTCCAGTGTATATTGGGATATGAAGTGCAGAAAGAAGGTGTTCCACA
CAATTGTGTTCATGATGCAGAAGCTGCAATGAAACTTGTACTTGCTATACTAGATAATGG
AGCAGAGACCTCCGTTCCACTATCTAAAGAGGTAAGTTGGATTGCACAAACTTCATTAGT
TCAATCTAAACTTATAAAAGCATAACGTTTTGTCTGCATAATTCCATATAGATGTTGGAA
GCTGAGAAGTCAAAGCTCTACCTTCATAGAATCCCTTGCAATGTTCCATATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 57-K118543-0022-076-C06C-8409
GenBank Accession LN509549 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118543-0022-076-C06C-8409
TATATGGAACATTGCAAGGGGATTCTATGAAGGTAGAGCTTTGACTTCTCAGCTTCCAAC
ATCTATATGGAATTATGCAGACAAAACGTTATGCTTTTATAAGTTTAGATTGAACTAATG
AAGTTTGTGCAATCCAACTTACCTCTTTAGATAGTGGAACGGAGGTCTCTGCTCCATTAT
CTAGTATAGCAAGTACAAGTTTCATTGCAGCTTCTGCATCATGAACACAATTGTGTGGAA
CACCTTCTTTCTGCACTTCATATCCCAATATACACTGGAAATCAAATCAAATAAACAATG
TGATACATCGGTCATGTACATACGAGATTCCATATCTCATGAGCAGAGGGTTGAGCTTTC
ACAGGCTTTACCTTGCACAGATAATTCAAAGAAGGTCTTTTAAGTCTTAAAGGCCTTCTT
GTACCGTCATAGTTATACTTAAACACAAGTGAAGTATCAATTACTCT

Last Updated on 06.12.2018 16:11