Sequences from confirmation amplicon


Confirmation sequences for line 077A11 and hit At1g65590 are shown.
Line ID 077A11
Locus At1g65590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24389615
Primers used for PCR 03jg - 8474, show primer details
   
Sequence Name 14-K113214-0022-077-A11C-8409
GenBank Accession LN492582 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113214-0022-077-A11C-8409
TTTCTTCATCTTCAACTTTTTTTTATCTTCTCTAATATATTTCGCATTTTCGACGATAGT
AACTATGATAATATTCATTGTTATAAATAAAGGGGAAAGGGATATCCTGCCTTGTGGCCC
TCCAAGAATTGTCAAGAACCACTTGACGTGAGCAGTGACTTCACATTCAAAGTCATTGAT
GGCATTCTCTCTGGTAAAAAGCTTTAAAACACTTTTTGCTTTATGTATGCATCACCAAAT
GTTGCCTAACTTCTTATGGATATATTTCATTCAGATTTCAGCAAGATCTTTAAGTTTAAA
TTTGTCCACTTGGGTGGTGATGAAGTAAATACAAGTAATGATCAATATCTCTTTTGTTTA
TCATATATCCTATCTATGGTGTTGGTTTTGATTACATGTACTTCGTTGCAGCTTGTTGGT
CTGCAACACCGCGAATAGCCCAATGGTAATGATACTCAGACACTTGCATAGTTCTTTAAA
ACGTTTTTCCTCATGTTACTCCAACTTTCTAACTTTGAAGGCTTAAAAAACATCGGATGA
GTGAAAAAGAAGCCTATCAATATTTCGTGTTGCGGGCGCAAAAAATCGCCTTGTCTCACG
GATATGAAATTATCAACTGGTAATTAAATTACACTTTCCTTACAAAATGAAAACGACAAG
TAATCAAAATTTTCATATGACTTACTACATTTTTGTCTAATGTTATACTGTAGGGAAGAG
ACCTTCATCAATTTTGGAAGCAAATTAAACCGGAAAACCGTGGTTCACAACTGGTAATTG
CATCTGTACGTAGAAAAGAAGTACAAATTGTGACTCGACGTTGAATTAATATAACGTTTT
CCTTTGA
   
Sequence Name 14-K113215-0022-077-A11C-03jg
GenBank Accession LN492583 [GenBank]
Sequencing primer gene specific primer, 03jg: 5'-CTGTGTTAAGCCTGATTCATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113215-0022-077-A11C-03jg
CGTCGAGTCCAATTTGTACTTCTTTTCTCGTACAGATGCAATTACCAGTTGTGAACCACG
GNTTTTCCGGTTTAATTTGCTTCCAAAATTGATGAAGGTCTCTTCCCTACAGTATAACAT
TAGACAAAAATGTAGTAAGTCATATGAAAATTTTGATTACTTGTCGTTTTCATTTTGTAA
GGAAAGTGTAATTTAATTACCAGTTGATAATTTCATATCCGTGAGACAAGGCGATTTTTT
GCGCCCGCAACACGAAATATTGATAGGCTTCTTTTTCACTCATCCGATGTTTTTTAAGCC
TTCAAAGTTAGAAAGTTGGAGTAACATGAGGAAAAACGTTTTAAAGAACTATGCAAGTGT
CTGAGTATCATTACCATTGGGCTATTCGCGGTGTTGCAGACCAACAAGCTGCAACGAAGT
ACATGTAATCAAAACCAACACCATAGATAGGATATATGATAAACAAAAGAGATATTGATC
ATTACTTGTATTTACTTCATCACCACCCAAGTGGACAAATTTAAACTTAAAGATCTTGCT
GAAATCTGAATGAAATATATCCATAAGAAGTTAGGCAACATTTGGTGATGCATACATAAA
GCAAAAAGTGTTTTAAAGCTTTTTACCAGAGAGAATGCCATCAATGACTTTGAATGTGAA
GTCACTGCTCACGTCAAGTGGTTCTTGACAATTCTTGGAGGGCCACAAGGCAGGATATCC
CTTTCCCCTTTATTTATNACAATGAATATNATCATAGTTACTATCGTCGAANATGCGAAA
TATATTAGAGAAGATAAAAAAAAGTTGAAGATGAAGAAATTACCATGAGAGAGCATGTCC
TGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAGAAAGAGTAA
TTACCAATTTTTTTTCATTCAAAATGT

Last Updated on 06.12.2018 16:11