SimpleSearch - Line and FST details
Line specific information
| Line ID | 077A11 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N407307) |
| Segregation Analysis | 50:49:41 |
| Confirmed for Hit | At1g65590 |
| Parent of DUPLO pair | 488 |
| Parent of pair(s) | none |
| Note |
Gene hit At1g33710
| Sequence (A. th genome BLAST matches underlined) | >81-K016172-022-077-A11-8409 TATCCTGAAATTTCCCGGACTGAAGCCTTTACATTGAATATATCCTGGTGACTCTGTATG AACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCC ATGGGAATTGGGGATCCTCTAGGGAAATAATCACGATCGTTAGGCCTGCTGTAACCATTA CAAGATTAGACTCTATGATATCCCTGATCATCAACACTAAATAAAACTCTCCTATCCTAG GGCTGAACTAATGCAAACCTTCATGTACTGCTTACTGTGGGAGACCCACTTAGTCTCCGA ATCGACCCATACTGGATGGAAGACTATAGAATGCTTATATATGAGATCCCAGGCAATTGA TCATATACACATTAACCTTGATCTCCACCAGGCCAGCATCTCCATGCCTGAGATATAAGA CACGGTCTCTATCCTTCTTCGACAGACGCATATGGAATGTCCNAAAATTAGCGAGAACAT TTTCCTATGGGATCTCCCCNAATAGGGAGAANNAGNAANAANGCTGGGATAGCATCTACT TCCTAATATCTCCAATTGCCGCACGACACCTATTTTACAAAAGAAGGTACCCCGAGGATC GATCGCTATGGTATGCTCCCNTAAAAAAACACAACCCATGAAGGCGTTCCCCACAACAAA GCTGAAAGCAGGCAGCTCCAATTCCCACCAACAGGGGGCCAAAAAAAACACCAAATTCCC AAGGCAAAAAAAAATGGGCCCCTGCCCTCCATGGCCAAAGAACAAAACCCCCAAAGGCGT TTGTAATTGCCTACCCCCAAAGGCAGGCCTATGGATCTCCCCCAAAAGGGAAGAACCCNC ACACCAGCCGCCCCTCGGGACTACACCCGGAAGATAAATAGGCGA |
| GenBank Accession | FR802287 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:12220062 - go to primer design |
| BLAST e Value | 2e-08 |
| Hit Clone Code (BAC ID) | F14M2 |
| Hit Gene Code | At1g33710 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RNA-directed DNA polymerase (reverse transcriptase)-related family protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g65590
| Sequence (A. th genome BLAST matches underlined) | >81-K016171-022-077-A11-8409 TGATATGTAATTTCCCGGACTGAAGCATTTACATTGAATATATCCTGGTGACTCTGTATG AACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCC ATGGGAATTGGGGATCCTCTATGGGATCCTCCCTTATAGTGAGAGGANNCAAATATTACT TGATGGCATGTGTATGATTGAGTATAACAACTTAGACCACTATCCTATCTTAAAAGCCTA AGTACATTTTCCTTGTTAGAATACACAAAGCCAAAAACTGATAACGACTTATGCTAAACC ATGAAAGCTTTGAGAAACAATAATAAGGTTGGTTAGTGTTTTGTAGTCAAATATGACTTG ATGTCATGTGTATGATTGATTATTATAACTTAAACCGCAACTTGATCTTTTAAGCCTAAG TAGTGTTTCCTTGTTAGAACAAACAAATCCAAAGACTCATACAGACTTTGTCTACACCAT GAAATGATTGACAAACTATGGATCTCCCTTTTAAAAAGAGGGATTCCCNCATGGTTACTC CAACTTTCTAACTTTTGAAGGCTTTAAAAAACATCGGATGAGTGAAAAAAGAAGCCTATC CATTATTTTCGTGTTGCGGGGCGCAAAAAATCGCCCTTGTCTCACGGATATGAAATTATC AAACTGGTAATTAAAATTACACTTTTCTTTACAAAATGAAAACGACCAGTAATCAAAATT TTCCTATGACTTACTACATTTTTTGGCTAATGTTATACTGAAGGGAAGAAACCTTCATCC ATTTTTGAAAACAAATTAAACCGGAAAACCGGTGGTTCCAAACTGGTAATTGCATCTGTA CGT |
| GenBank Accession | FR802286 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:24390109 - go to primer design |
| BLAST e Value | 1e-84 |
| Hit Clone Code (BAC ID) | F5I14 |
| Hit Gene Code | At1g65590 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | beta-hexosaminidase 3 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit T2N12
| Sequence (A. th genome BLAST matches underlined) | >81-K016171-022-077-A11-8409 TGATATGTAATTTCCCGGACTGAAGCATTTACATTGAATATATCCTGGTGACTCTGTATG AACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCC ATGGGAATTGGGGATCCTCTATGGGATCCTCCCTTATAGTGAGAGGANNCAAATATTACT TGATGGCATGTGTATGATTGAGTATAACAACTTAGACCACTATCCTATCTTAAAAGCCTA AGTACATTTTCCTTGTTAGAATACACAAAGCCAAAAACTGATAACGACTTATGCTAAACC ATGAAAGCTTTGAGAAACAATAATAAGGTTGGTTAGTGTTTTGTAGTCAAATATGACTTG ATGTCATGTGTATGATTGATTATTATAACTTAAACCGCAACTTGATCTTTTAAGCCTAAG TAGTGTTTCCTTGTTAGAACAAACAAATCCAAAGACTCATACAGACTTTGTCTACACCAT GAAATGATTGACAAACTATGGATCTCCCTTTTAAAAAGAGGGATTCCCNCATGGTTACTC CAACTTTCTAACTTTTGAAGGCTTTAAAAAACATCGGATGAGTGAAAAAAGAAGCCTATC CATTATTTTCGTGTTGCGGGGCGCAAAAAATCGCCCTTGTCTCACGGATATGAAATTATC AAACTGGTAATTAAAATTACACTTTTCTTTACAAAATGAAAACGACCAGTAATCAAAATT TTCCTATGACTTACTACATTTTTTGGCTAATGTTATACTGAAGGGAAGAAACCTTCATCC ATTTTTGAAAACAAATTAAACCGGAAAACCGGTGGTTCCAAACTGGTAATTGCATCTGTA CGT |
| GenBank Accession | FR802286 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr4:3951704 - go to primer design |
| BLAST e Value | 4e-93 |
| Hit Clone Code (BAC ID) | T2N12 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
|
Last Updated on 10.06.2021 13:37 |
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