Sequences from confirmation amplicon


Confirmation sequences for line 077C04 and hit At5g55230 are shown.
Line ID 077C04
Locus At5g55230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22404762
Primers used for PCR CL53 - 8474, show primer details
   
Sequence Name 42-K032926-022-077-C04C-CL53
GenBank Accession LN504362 [GenBank]
Sequencing primer gene specific primer, CL53: 5'-TTTCAGGTGTTTCATAGAGCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032926-022-077-C04C-CL53
CACATCTTTTTCATTTTTTTATTCATCATGGTGAAGCTGGAACTTGATGATCTGCAGCAC
CAGAAACTGGAGATGAAGCAGCTTCCTCTTTCGAAATGGCAACGTAGTTTGTAGGAGCAG
CCGGCCTGTTGAGAGTCTCCCTTCTCCCTGCTTCTTTTGCAGTAGACCTGCTTCCATTCT
GGTTTGCATTCAAAGATAAACGCCGGTTATGTGTTCCATTGGCTCCTCCGTTGTTAGCTC
GTGGCCCCACCGTTTTCTTAGCACTGACCGGTCTTGCAGGGCTTGGCCTGGTGCTAAAGG
CAGATTCTTGCTCTACGTGTGGCTGTTCTTGAACCTTCTTTTGTTCCTGCTCATTAAGAC
AAAGAGGAGTTTAGGGAGTGACTGACATTCTCGCCTTATCATTAATCGAGTAGAAATGAT
GAACTTACCCTCAGCCTCCGTTTCTCGCATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 42-K032926-022-077-C04C-8409
GenBank Accession LN504361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032926-022-077-C04C-8409
TTACAATTGAATATGCGAGAAACGGAGGCTGAGGGTAAGTTCATCATTTCTACTCGATTA
ATGATAAGGCGAGAATGTCAGTCACTCCCTAAACTCCTCTTTGTCTTAATGAGCAGGAAC
AAAAGAAGGTTCAAGAACAGCCACACGTAGAGCAAGAATCTGCCTTTAGCACCAGGCCAA
GCCCTGCAAGACCGGTCAGTGCTAAGAAAACGGTGGGGCCACGAGCTAACAACGGAGGAG
CCAATGGAACACATAACCGGCGTTTATCTTTGAATGCAAACCAGAATGGAAGCAGGTCTA
CTGCAAAAGAAGCAGGGAGAAGGGAGACTCTCAACAGGCCGGCTGCTCCTACAAACTACG
TTGCCATTTCGAAAGAGGAAGCTGCTTCATCTCCAGTTTCTGGTGCTGCAGATCATCAAG
TTCCAGCTTCACCATGATGAATAAAAAAATGAAAAAGATGTGTAATTTTCAGTGTTTGAG
TTGGTTTAGGAAGAAAAGGCTCTATGAAAAANCCTGAA

Last Updated on 06.12.2018 16:11