Sequences from confirmation amplicon


Confirmation sequences for line 077H06 and hit At1g19490 are shown.
Line ID 077H06
Locus At1g19490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6751982
Primers used for PCR 02yd - 8474, show primer details
   
Sequence Name 24-K119741-0022-077-H06C-02yd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02yd: 5'-GCTAAAACTATTACCACGCAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119741-0022-077-H06C-02yd
GGCAAAACTCCTTTACGTTGTACCTTGCCCTTGGTTTCTCCCTCCTCCTGATCACAGCAA
TGGTGTTCCTTTCGGGCTTCAAGATACACAAAGAGGTACTTTTTCAAACGGGCACCATAT
CGATGATTCTTCTGCAAGACCGATGGATGTCACAGAAACTCCGCGGTCCCATCTACCAAC
TAGAATCAAAGAAGAGGACTCTGGTTCACCCGAAACCAGACCTTTATATGATCTCAACGA
ATCTGCTACTGAAGTCCTCTCAGAAGGAGGAGATGGATTTCCTGTAACGCAGCAAGCTTA
TAGTTTAAAGCATGAAGATGTTTCTGAGACAACTAATGGAGTTACACTGATGCCGCCTGG
TCATCATGTTTTGATTTCTCTACCGGAGAAAAAGCATGGATCGTTGGCTGCAGCGGAAGC
TAGAAAGAGACGGAAAGAACTCACAAGGCTCAAGAACCTCCATGTCCGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
CGATTATT
   
Sequence Name 12-K119740-0022-077-H06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K119740-0022-077-H06C-8409
TCATTGAATATATCCGGACATGGAGGTTCTTGAGCCTTGTGAGTTCTTTCCGTCTCTTTC
TAGCTTCCGCTGCAGCCAACGATCCATGCTTTTTCTCCGGTAGAGAAATCAAAACATGAT
GACCAGGCGGCATCAGTGTAACTCCATTAGTTGTCTCAGAAACATCTTCATGCTTTAAAC
TATAAGCTTGCTGCGTTACAGGAAATCCATCTCCTCCTTCTGAGAGGACTTCAGTAGCAG
ATTCGTTGAGATCATATAAAGGTCTGGTTTCGGGTGAACCAGAGTCCTCTTCTTTGATTC
TAGTTGGTAGATGGGACCGCGGAGTTTCTGTGACATCCATCGGTCTTGCAGAAGAATCAT
CGATATGGTGCCCGTTTGAAAAAGTACCTCTTTGTGTATCTTGAAGCCCGAAAGGAACAC
CATTGCTGTGATCAGGAGGAGGGAGAAACCAAGGGCAAGGTACAACGTAAAAGTGAGTTT
TTTGCCCATTATCATCTGCAGCATTTTCATGCTCCTGCGTGGTAAAGNTTTTAG

Last Updated on 06.12.2018 16:11