Sequences from confirmation amplicon


Confirmation sequences for line 077H07 and hit At4g21960 are shown.
Line ID 077H07
Locus At4g21960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11647395
Primers used for PCR AH33 - 8474, show primer details
   
Sequence Name 11-K105456-0022-077-H07C-AH33
GenBank Accession LN491109 [GenBank]
Sequencing primer gene specific primer, AH33: 5'-CAAACCCTTGTTGTCTAGGATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105456-0022-077-H07C-AH33
GGGTGCCCGGTCGTTACGCACGTACTGGACCGCCTTTGGGTCAGGGATCGAGTCAGGACA
CTTGTGTAGCATGTGAGGGACGTGGTCCGGGTTTAAGGACGGGTCCACCTCAGGGTACAA
ACGATGGACCAGCTTGACACAGTGAGTTCTTCCCACGCTGTGTGACCCTGCACGTGTCAT
AAAAAAAAAAAGTCAAAATCTTTCAGTATCGAACTTAAAAATCTAACAAAAAACTTAAAT
TCCAATCGCCTAAATTCAAATCCCATAAAAGTAAAGTCAATTAGTTTGATTAGTATGTTT
AAGCATGCATGATTTTATTGACCAAAATAACCTTGTGCTAGGCTATTGTACCGGGGCATA
AACAAGGATTATCCTGTCTTATTTGTTAGTACTAGTGATAAATCGGATAAGGATAACTTT
CAAAGTTCAACTCAAATCGCATACCCTCCTTTTGTGATTCAATAAAGTTAGCTTTAGTAG
TGGTTCATTAGTCATAAAAGTGTTTCATGCATGAGTAAATTGACTGAAATACCCTTAATC
CCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATG
AGTATGATGGTCAATATGGAGAAAAAAAAAGAGTAATTACCATTTTTTTTTCAATTCAAA
AATGTAAATGTC
   
Sequence Name 27-K105457-0022-077-H07C-8409
GenBank Accession LN498384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105457-0022-077-H07C-8409
GCCATTTACATTGAATATATCCTGGGATTAAGGGTATTTCAGTCAATTTACTCATGCATG
AAACACTCTTATGACTAATGAACCACTACTAAAGCTAACTTTATTGAATCACAAAAGGAG
TGTATGCGATCTGAGTTGAACTTTGAAAGTTATCCTTATCCGATCTATCACTAGTACTAA
CAAATAAGACAGGATAATCCTTGTTTATGCCCCGGTACAATAGCCTAGCACAAGGTTATT
TTGGTCAATAAACTCATGCATGCTTAAACATACTAATCAAACTAATTGACTTTACTCTTA
TGGGATCTGAATTTAGGCGATTGGAATTTAAGTTTCTTGTTAGATTTTTAAGTTCGATAC
TGAAAGATTTTGACTTTTTTTTTTTATGACACGTGCAGGGTCACACAGCGGGGGAAAAAC
TCACTGTGTCAAGCTGGTCCATCGTTTGTACCCTGAGGGGGACCCGTCCTTAAACCCGGA
CCACGTCCCTCACATGCTACACAAGTGTCCTGACTCGATCCCTGACCCAAAGGCGGTCCA
GTACGTGCGTAACAACCGTGGCACCCCCATGGTGCTAGACAACAATTACTACCGTAACAT
CCTAAAC

Last Updated on 06.12.2018 16:11