Sequences from confirmation amplicon


Confirmation sequences for line 078B06 and hit At5g13610 are shown.
Line ID 078B06
Locus At5g13610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4383764
Primers used for PCR 22ay - 8474, show primer details
   
Sequence Name 70-K106975-0022-078-B06C-8409
GenBank Accession LN512398 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106975-0022-078-B06C-8409
TCGGACTGAGCCATTTACATTGAATATATCCTGAAACATCCATAGAAGAATCAACATTTC
ACAATGAGAAGGTACCTCAGCTTCATAAAACTGAATAAAGGTATAAGATTGAACTTACAG
TGGGATCAGAATGATTGCCAAACTTAAGAACAACATAGTTAGTCATACGCGAAGTAGGTG
GGATGAAGTTCTGCTTGTTCTGCTCTATTAAGCTTCTCAAATCAACGCTGCACACAATTT
ACAATCCAAAAACTACTTCAATCATCAAAACACATTGGCAAATACATCCACTTTTAAAGT
TTGTGAACAAAATTATACCTAGTGGAGAAGAAATAAGCTCGCACAGGAATAGAAAGCTTG
GCTTCGTCCTCTAAGCCCATAGAAATCTCTTCATCAACCTCAGCTGGATGCTGATTCTGA
TTCTGAAGCTGCTGCGGCTGAGCAGAAGCGACGGGGGTCGTCGTAGACGAAGAGATAGCC
CTCTCAG
   
Sequence Name 86-K106977-0022-078-B06C-22ay
GenBank Accession LN514659 [GenBank]
Sequencing primer gene specific primer, 22ay: 5'-CTGAGAGGGCTATCTCTTCGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K106977-0022-078-B06C-22ay
GCAGCAGCTTCAGAATCAGAATCAGCATCCAGCTGAGGTTGATGAAGAGATTTCTATGGG
CTTAGAGGACGAAGCCAAGCTTTCTATTCCTGTGCGAGCTTATTTCTTCTCCACTAGGTA
TAATTTTGTTCACAAACTTTAAAAGTGGATGTATTTGCCAATGTGTTTTGATGATTGAAG
TAGTTTTTGGATTGTAAATTGTGTGCAGCGTTGATTTGAGAAGCTTAATAGAGCAGAACA
AGCAGAACTTCATCCCACCTACTTCGCGTATGACTAACTATGTTGTTCTTAAGTTTGGCA
ATCATTCTGATCCCACTGTAAGTTCAATCTTATACCTTTATTCAGTTTTATGAAGCTGAG
GTACCTTCTCATTGTGAAATGTTGATTCTTCTATGGATTGTTTCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 14bd - 8474, show primer details
   
Sequence Name 42-K118893-0022-078-B06C-14bd
GenBank Accession LN504652 [GenBank]
Sequencing primer gene specific primer, 14bd: 5'-AATTCTGCTACCATACCATCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118893-0022-078-B06C-14bd
AGTTATTCTCTTTTCATTTTTCCCATTCCAGAATACAATGTGTAAGGATTTGACATGGTT
ACCTGCCGGCCATAGTAATCCAGAGCAATACTCTGGCCCAGAACACATCCTATGGTTCTG
ATCCCATCAGTATTCAAGAACTGCAACCTGATAAAATCCCGTCCTACCTCCATCCATGTG
TCCAGATTTGGATTCTCTCTTACTTCATATTCTACAGTTTTACAGAACCAGAGATATGAG
AAAGTAAGCAACATTGCGAAAACAGCACACAGCGCAAAAGAAAAGCAAGCAACTCACCAT
CTTTTCTCATTTCTGGAAGCAACCCAGAGGCATGCCTCTCCACTACTTTAAGATATTCAT
CAACCTCATGTTCCCTTACATTAAACAAGACAATCGATCCATAATGAAATACTACCATGT
AAATAGACTCACTCCCACTTATGCGTCCTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K118892-0022-078-B06C-8409
GenBank Accession LN487253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118892-0022-078-B06C-8409
TCGGATGAGCATTTCATTGAATTATCCTGAGGACGCATAAGTGGGAGTGAGTCTATTTAC
ATGGTAGTATTTCATTATGGATCGATTGTCTTGTTTAATGTAAGGGAACATGAGGTTGAT
GAATATCTTAAAGTAGTGGAGAGGCATGCCTCTGGGTTGCTTCCAGAAATGAGAAAAGAT
GGTGAGTTGCTTGCTTTTCTTTTGCGCTGTGTGCTGTTTTCGCAATGTTGCTTACTTTCT
CATATCTCTGGTTCTGTAAAACTGTAGAATATGAAGTAAGAGAGAATCCAAATCTGGACA
CATGGATGGAGGTAGGACGGGATTTTATCAGGTTGCAGTTCTTGAATACTGATGGGATCA
GAACCATAGGATGTGTTCTGGGCCAGAGTATTGCTCTGGATTACTATGGCCGGCAGGTAA
CCATGTCAAATCCTTACACATTGTATTCTGGAATGTGGAAAAATGAAAAGAGATATAACT
GTGTGTGAGCAATCTGTTTCTGTTATAGGTTGATGGTATGGAGGCAGAAT

Last Updated on 06.12.2018 16:11