Sequences from confirmation amplicon


Confirmation sequences for line 078C02 and hit At3g12230 are shown.
Line ID 078C02
Locus At3g12230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3900214
Primers used for PCR 10ol - 8474, show primer details
   
Sequence Name 20-K114734-0022-078-C02C-10ol
GenBank Accession LN495323 [GenBank]
Sequencing primer gene specific primer, 10ol: 5'-TGCAAATGGAAACAATAACAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114734-0022-078-C02C-10ol
TATACCCGGCTACTTGATCGAGTATCATCCATGGCCTCCATTTTTCAGTAATGGAATAGT
TGAGAGATCTTATCCATGCTTGAGTTCCAAGGAAAGGGATTGTCATATCGTGATCACCGC
TACTCAACAGAAAGACCATGCCAGAAAAAAAAAAGCTTACAAAAAAACTAATTAATGAAA
GCACAAGCTTGTAAATCAGTTTAATTTTGTGTACCTAATGACTAGAGATCGATAGCCTTT
GATGCTGTTATTCATATGGTACGGTATGCTGCTTTTAATGTCTTGATTGCTACGCACGCT
TAGATCACATCGTTCCCATTTCCCTTTACTCCCCTGAGATCAAAATATTCTTAGAGTTTG
ATAAACAGATTTTACCAACGATCTAATCATAAACTTGTCATATACCTCCACAACTTTGAG
TGCTCTGCGGACAATCTCGTTATTTGCCCAATATATAGATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATTTCCGCA
   
Sequence Name 36-K114733-0022-078-C02C-8409
GenBank Accession LN502330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114733-0022-078-C02C-8409
CATCTATATATTGGGCAATAACGAGATTGTCCGCAGAGCACTCAAAGTTGTGGAGGTATA
TGACAAGTTTATGATTAGATCGTTGGTAAAATCTGTTTATCAAACTCTAAGAATATTTTG
ATCTCAGGGGAGTAAAGGGAAATGGGAACGATGTGATCTAAGCGTGCGTAGCAATCAAGA
CATTAAAAGCAGCATACCGTACCATATGAATAACAGCATCAAAGGCTATCGATCTCTAGT
CATTAGGTACACAAAATTAAACTGATTTACAAGCTTGTGCTTTCATTAATTAGTTTTTTT
GTAAGCTTTTTTTTTTCTGGCATGGTCTTTCTGTTGAGTAGCGGTGATCACGATATGACA
ATCCCTTTCCTTGGAACTCAAGCATGGATAAAATCTCTCAACTATTCCATTACTGAAAAA
TGGAGGCCATGGATGATACTCGATCAAGTAGCCGGGTATATATATAACCATTTCTTTTTC
ACCTCCTCTTTTTGTTA

Last Updated on 06.12.2018 16:11