Sequences from confirmation amplicon


Confirmation sequences for line 078F06 and hit At3g05550 are shown.
Line ID 078F06
Locus At3g05550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1612954
Primers used for PCR CW64 - 8474, show primer details
   
Sequence Name 02-K104226-0022-078-F06C-CW64
GenBank Accession LN487013 [GenBank]
Sequencing primer gene specific primer, CW64: 5'-AAGTGCTTCGTACAGTACCATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104226-0022-078-F06C-CW64
GGCTAGACATAAAATTTATGTCGATACAACTATAGCTTACAGGCAAGAACAGAGAAATGT
ACATGCTTTTGGGACATGGAGATGATGATGTTGCTCAACTATAAATGTTCATGGCTACAG
AAAATGTTTCAAAATGCAAATGAGACTCAAAAACTTTACAAAAAAATCATTCAACCATTT
ATAATCAATCAATCCTTTTGATTAGAAGATTCACCTGGCGTGGATGATCTTGACACTGGT
TTTCATGGCAGGTTGGGACCAGTTATAGGCGATAGAACCGGTAATACCACTTAGCCATAA
ACAACCTGATTCAACAACGGTCAAATTAAAACAATACAGGTTCAGAATTAGCAGAAAGAA
ATATCAAAACACCAAAAATAAAGTAGATGGGAATGCATTCTCTAATATCTTTGCATGTCA
AAGAACATTAAGAATTGAGGACCAAAAATGTCTCAGTGGAATCAAGAGGAAATCCAAGAC
GGTAACAGAAACCGTAACAAGTTAATAATCAATTCTACTATATGACATTAAGGCGATGAA
ATCTAAGATCACAATAAACGACCCANGAGAAAAGGAATGTTGAGAAGACAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 18-K104227-0022-078-F06C-8409
GenBank Accession LN494285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104227-0022-078-F06C-8409
GCCATTTACAATTGTCTTCTCAACATTCCTTTTCTCTTGGGTCGTTTATTGTGATCTTAG
ATTTCATCGCCTTAATGTCATATAGTAGAATTGATTATTAACTTGTTACGGTTTCTGTTA
CCGTCTTGGATTTCCTCTTGATTCCACTGAGACATTTTTGGTCCTCAATTCTTAATGTTC
TTTGACATGCAAAGATATTAGAGAATGCATTCCCATCTACTTTATTTTTGGTGTTTTGAT
ATTTCTTTCTGCTAATTCTGAACCTGTATTGTTTTAATTTGACCGTTGTTGAATCAGGTT
GTTTATGGCTAAGTGGTATTACCGGTTCTATCGCCTATAACTGGTCCCAACCTGCCATGA
AAACCANGTGTCAAGATCATCCACGCCAGGTNGAATCTTCTAATCAAAAGGAANTTGATT
GATTATAAATGGTTGAAATGATTTNTTTTGTAAAGTTNTTTGAGTCTCANTTTGCATTNT
TGAAACANNTTTTCTGTAGCCATNGAACANNTTTATAGTTGAAGCAACANNTCATCATCT
CCATNGTCCCAAAAGCATGGTACATTTTTCTCTGTTCTTGNCCTGTAAGCCCTATAGTTG
TTATNCGACATNNAAATTTTATNNGTTCTTAGCCAANNATCATTTGTTCGAAGACATNCC
AGTCACATNGGTACTGTACGAAAAGCAC

Last Updated on 06.12.2018 16:11