Sequences from confirmation amplicon


Confirmation sequences for line 078F12 and hit At3g06460 are shown.
Line ID 078F12
Locus At3g06460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1983423
Primers used for PCR YN06 - 8474, show primer details
   
Sequence Name 41-K026964-022-078-F12C-8409
GenBank Accession LN503942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K026964-022-078-F12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAAACACACCATTGAA
ATAAACTGTCCAAATCCCGGCGCAACCCGACCCGAAATAATGCTCTGGGAGCATCCAAGC
GGCTCCTAACCCCATGCCGAAAGCAAACTGAACCATTTGAAAATTCGTCACCAACTTCTT
CCACTTGGGCCTCGATCCGATAGCGCAGAGGAAATAGTACCCGTACATAATCACATGGAC
CGTCGAGTTCAACACGAGCCCAACAGGAAACATCGATTGACGTGTTCGTAACCAGAGGTA
GCACAAAATCACAACCGTTGCGTGGTGGTAGACGTGGAGGAACGAGAGCCGTTGGATTGA
TTTGTTGAGTATGATGAGAAGTGTGTCTACGAACTCAAGGATCTTCGAGAGGTAAAAGAC
TTGAGC
   
Sequence Name 45-K026964-022-078-F12C-YN06
GenBank Accession LN505401 [GenBank]
Sequencing primer gene specific primer, YN06: 5'-TTTCTTTTGGGCTCAAGTCTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K026964-022-078-F12C-YN06
CTCTCGAAGATCCTTGAGTTCGTAGACACACTTCTCATCATACTCAACAAATCAATCCAA
CGGCTCTCGTTCCTCCACGTCTACCACCACGCAACGGTTGTGATTTTGTGCTACCTCTGG
TTACGAACACGTCAATCGATGTTTCCTGTTGGGCTCGTGTTGAACTCGACGGTCCATGTG
ATTATGTACGGGTACTATTTCCTCTGCGCTATCGGATCGAGGCCCAAGTGGAAGAAGTTG
GTGACGAATTTTCAAATGGTTCAGTTTGCTTTCGGCATGGGGTTAGGAGCCGCTTGGATG
CTCCCAGAGCATTATTTCGGGTCGGGTTGCGCCGGGATTTGGACAGTTTATTTCAATGGT
GTGTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11