Sequences from confirmation amplicon


Confirmation sequences for line 078G07 and hit At4g12000 are shown.
Line ID 078G07
Locus At4g12000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7194807
Primers used for PCR BH90 - 8474, show primer details
   
Sequence Name 43-K104418-0022-078-G07C-BH90
GenBank Accession LN504828 [GenBank]
Sequencing primer gene specific primer, BH90: 5'-CTCATCTTTCTCCAAAATCACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K104418-0022-078-G07C-BH90
CATCCTTCCTTCTCCCCAGGTTTCTTTCGATTCGTTGAGTCATTCAATTTTTCCATCACT
GGGTTTTTCTCTCTGAATCCGATCGGAGAATCCAGTCGATTACTAATCTAGCGCTCTCTT
TTTTTCTACTCGATGACTTATCACGAAGAAGACGATACAGTTTCTGAATTTCGAGTTAGA
GTTGAAGAAGATGGAGTTGATAAATTAGGTCATTATGTTAAACTTACTGAAGATTTCGAA
GTACATCGACAAGAAACAGAACAAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCGCGGTTATT
   
Sequence Name 59-K104419-0022-078-G07C-8409
GenBank Accession LN509993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K104419-0022-078-G07C-8409
GCCATTTACAATTGAATAATTCTTGTTCTGTTTCTTGTCGATGTACTTCGAAATCTTCAG
TAAGTTTAACATAATGACCTAATTTATCAACTCCATCTTCTTCAACTCTAACTCGAAATT
CAGAAACTGTATCGTCTTCTTCGTGATAAGTCATCGAGTAGAAAAAAAGAGAGCGCTAGA
TTAGTAATCGACTGGATTCTCCGATCGGATTCAGAGAGAAAAACCCAGTGATGGAAAAAT
TGAATGACTCAACGAATCGAAAGAAACCTGGGGATGAAGGAAGGATTGAGAATAACAAAT
AAAGAAGATTTGTGTGATTTTGGAGAAAGATGA

Last Updated on 06.12.2018 16:11