Sequences from confirmation amplicon


Confirmation sequences for line 078G08 and hit At3g51310 are shown.
Line ID 078G08
Locus At3g51310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19048204
Primers used for PCR MK74 - 8474, show primer details
   
Sequence Name 21-K101424-0022-078-G08C-8409
GenBank Accession LN495597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K101424-0022-078-G08C-8409
GCCTTTACAATTGAATATATCCTGGATACAGAAAGTCTCAACTCAGGTTCAGCTCAGTAG
GAAAAAATCACAAAGAGTAAAATAGCAGCATGACCAGACATTGGACTCTCACAGTACGCA
AACATATTCAATACATACATGCTGGATATGAGAGCAGTATATCATTAGCATTTCCAACCA
ACCTGATGTTGCATTCGAACCCATAATTTGTTCATCTCGGTAAAATTTTGAAGCACGAAC
TCTAAAGCATTCATGTGTGCATCACCATCTCTGGAGAGAAAAGCAAAACACTTGCCTCTT
AACATGTGTTTCATAGCTTAAACAAAATCATCTAACAGCAGGTCGTGTGGCCTTACCCTT
CCAAGTCAGAACCAATGCTGGGTAACTTATCCCTAGTAACTTGAGCAAGATAACTTCTCA
AGAAAAGACCACGCANGGGATGTTGAACAGCTCGACACATTTCAACAAGATCCTTAAGTA
TATCCGTAGCAGTAACATCCTTGGATTTGATATATACAGATCCTATGGTACATAANAGAT
ACCTGANACTCANTTGATGAGTATCTACNAAGTTAAAATCAGAGTGTGCAATTGAC
   
Sequence Name 61-K101427-0022-078-G08C-MK74
GenBank Accession LN510469 [GenBank]
Sequencing primer gene specific primer, MK74: 5'-GTTGTCATTTGCACACTCTGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K101427-0022-078-G08C-MK74
CATTGAGTTTCGGTATCTTTTATGTACCATAGGATCTGTATATATCAAATCCACGGTTGT
TACTGCTACGGATATACTTAAGGATCTTGTTGAACTGTGTCGAGCTGTTCAACATCCCTT
GCGTGGTCTTTTCTTGAGAAGTTATCTTGCTCAAGTTACTAGGGATAAGTTACCCAGCAT
TGGTTCTGACTTGGAAGGGTAAGGCCACACGACCTGCTGTTAGATGATTTTGTTTAAGCT
ATGAAACACATGTTAAGAGGCAAGTGTTTTGCTTTTCTCTCCAGAGATGGTGATGCACAC
ATGAATGCTTTAGAGTTCGTGCTTCAAAATTTTACCGAGATGAACAAATTATGGGTTCGA
ATGCAACATCAGGTTGGTTGGAAATGCTAATGATATACTGCTCTCATATCCNGCATGTAT
GTATTGAATATGTTTGCGTACTGTGAGAGTCCAATGTCTGGTCATGCTGCTATTTTACTC
TTTGTGATTTTTTCCTACTGAGCTGAACCTGAGTTGAGACTTTCTTGTATCCNGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCNATTCAAAAATGTAAA

Last Updated on 06.12.2018 16:11