Sequences from confirmation amplicon


Confirmation sequences for line 079A01 and hit At1g15670 are shown.
Line ID 079A01
Locus At1g15670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5390785
Primers used for PCR 05dl - 8474, show primer details
   
Sequence Name 18-K109936-0022-079-A01C-05dl
GenBank Accession LN494348 [GenBank]
Sequencing primer gene specific primer, 05dl: 5'-GGAAAGCTTCAGGACATAGTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109936-0022-079-A01C-05dl
GCTGGGTCGACCCGGTTAAGAACTCGTATCGGGTAATAAAACCATCCCCACCCCCGTGTA
CCGAATCTCGGTTCTTGAACTTGGTACCGGTCTAAGGAGCGAGCTGCCTCCGGTTCCAGG
TCATTCCAACGGGTTGCCTCTGTTCTGCAGATTAGTCTCTGTCGGGTCGGATCTTGTTGT
ATTGTGCGGGCTTGACCCGGTTACATGGCGTACCTCTGATTCGGTCTTCGTTTTCAGCTT
CTTGACCTCCACGTGGCGCGTCGGGAAGAGCATGCCAGGTGGACCGCGTTCCTTCTTTGC
CTGCGCTTCGGATTCTCAACGGAACGTCTTTGTGGCTGGAGGACACGACGAAGACAAGAA
CGCGATGATGTCAGCTCTGGTGTATGACGTGGCAGAAGACAGATGGGCTTTCTTGCCGGA
TATGGGCCGTGAGCGAGACGAATGCACAGCTATTTTCCACGCTGGCAAATTCCATGTCAT
CGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K109935-0022-079-A01C-8409
GenBank Accession LN487077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109935-0022-079-A01C-8409
TCGGACTGAGCCATTTCGATGACTGGAATTTGCCAGCGTGGAAAATAGCTGTGCATTCGT
CTCGCTCACGGCCCATATCCGGCAAGAAAGCCCATCTGTCTTCTGCCACGTCATACACCA
GAGCTGACATCATCGCGTTCTTGTCTTCGTCGTGTCCTCCAGCCACAAAGACGTTCCGTT
GAGAATCCGAAGCGCAGGCAAAGAAGGAACGCGGTCCACCTGGCATGCTCTTCCCGACGC
GCCACGTGGAGGTCAAGAAGCTGAAAACGAAGACCGAATCAGAGGTACGCCATGTAACCG
GGTCAAGCCCGCACAATACAACAAGATCCGACCCGACAGAGACTAATCTGCAGAACAGAG
GCAACCCGTTGGAATGACCTGGAACCGGAGGCAGCTCGCTCCTTAGACCGGTACCAAGTT
CAAGAACCGAGATTCGGTACACGGGGGTGGGGATGGTTTTATTACCCGATACGAGTTCTT
TAACCGGGTCGACCCGAGCTTGAGACAAGACAACAAGCTCTTGACTATGTCCTGAAGCTT
TCCA

Last Updated on 06.12.2018 16:11