Sequences from confirmation amplicon


Confirmation sequences for line 079B03 and hit At3g03830 are shown.
Line ID 079B03
Locus At3g03830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:980304
Primers used for PCR 03pq - 8474, show primer details
   
Sequence Name 33-K115290-0022-079-B03C-03pq
GenBank Accession LN501118 [GenBank]
Sequencing primer gene specific primer, 03pq: 5'-GCTAATTTCGTCCCATGTATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115290-0022-079-B03C-03pq
TGATAGATTCTGCCGACGACTATGACAGGGTTAGTCAGATGGATTTTCTTATATATCAAT
CAACGTAGCTAGAGAAACTTCTTCTACGGCCTTAACATGCGATCTTGTTCTCTTGGTCCT
ATGTGAGTACAGATTGTAATAGCTCTGCTTTAAATGTGATAACAAAACCATGTGAAAAAC
TTTGTCCTTGTGTGTCCCACATTGTTTTCAATGGATTAGTTTGATCTATATATATATACA
CATCTCCGCATCAGAGAGAATATCATCAACCACAAGCAATCCAGAACTTTAGTCCTTCCT
TTGATCCCATACATATTCAGAAGCTTCGAGAATCAAACTTCTAGACAAGAAAATGGCTTT
GGTGAGAAGTATCTTCAGTGCAAAGAAGATTCTTGGCGGCTCCTTAGCAAGAACGAGCAA
AGCACCAAAAGGTTTTCTTGCGGTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K115289-0022-079-B03C-8409
GenBank Accession LN490314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115289-0022-079-B03C-8409
TTGAATATATCCTGACACCGCAAGAAAACCTTTTGGTGCTTTGCTCGTTCTTGCTAAGGA
GCCGCCAAGAATCTTCTTTGCACTGAAGATACTTCTCACCAAAGCCATTTTCTTGTCTAG
AAGTTTGATTCTCGAAGCTTCTGAATATGTATGGGATCAAAGGAAGGACTAAAGTTCTGG
ATTGCTTGTGGTTGATGATATTCTCTCTGATGCGGAGATGTGTATATATATATAGATCAA
ACTAATCCATTGAAAACAATGTGGGACACACAAGGACAAAGTTTTTCACATGGTTTTGTT
ATCACATTTAAAGCAGAGCTATTACAATCTGTACTCACATAGGACCAAGAGAACAAGATC
GCATGTTAAGGCCGTAGAAGAAGTTTCTCTAGCTACGTTGATTGATATATAAGAAAATCC
ATCTGACTAACCCTGTCATAGTCTGTCGGCAGAATCTTTATCAGATTTTGACACAGATGA
GAAATTAATCAATACATGGGA

Last Updated on 06.12.2018 16:11