Sequences from confirmation amplicon


Confirmation sequences for line 079B12 and hit At5g50860 are shown.
Line ID 079B12
Locus At5g50860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20696220
Primers used for PCR 56zf - 8474, show primer details
   
Sequence Name 38-K125004-0022-079-B12C-56zf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56zf: 5'-ACCACTTTTCTTCAGGCCTTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K125004-0022-079-B12C-56zf
AATGTAAGGTGAAACCGCTCGTTACTTAATAAACTAGATACGGAAAACCGCCGTTTTTCA
CTCCTACGTGCAAAAGACTCGACTCCACTCAAAAACGCCGTCGTTTTACACAAAAACCGA
TTCTCACTCTCTCCAAGAGAAAAAAATCAATTTTTGAGCTCTTTCGGCGATCATGTTAAG
CTAAAGAGAGAAAGCTTCACCGATTCAAAATCATTTAGCCGATGGGATGCGTTTTATGCA
AAGAATCCACCGGAGACAAACGGAAACACAATAATCCCGATGAACCTCCTCCGGCGGACC
TCCGTAATACGGAGGATTTACCTTCTTCATCTTCAACCACCACCGCCGCCATTTCTGTCG
AAATCGGAGAGAAGAAGAAGAAAGATTTAGATTCGATTCAGATTCAGCCGGAGAGAAGAA
CATGGCACACTGGCGACTTCTCCGCCGGCTCTTCTCGCCGTCCGGGGATGAGTCTCCGTA
CGCCGGAAGGATGGCCACCTTGGCTTATTGCTGCTTGTGGCGATTCGATCAAAGACTTAA
CACCTCGCAGAGCTACCACATACGAAAAGCTCGAGAAGGTAGGTAACAAAAACACTAATC
ATTAATAAAGTAATAATCATTACTAGGTTTGGTATAATTAGAAAGTGTTTTGTTTTGTTT
TGATTAGTTTATGTTTGGTTTAATTTAATGAAGTAATGTTTTTGATTGTTATAGATTGGG
CAAGGAACGTATAGCAATGTGTACAAGGCGAAAGATTTATTGAGTGGGAAGATTGTAGCT
TTGAAGAAAGTTAGGTTTGATAATTTGGAAGCTGAGAGTGTTAAATTTATGGCTAGAGAG
ATTCTTGTGTTACGACGGCTTAATCATCCTAATGTTATTAAGCTTCAAGGTTTGGTTACT
TCACGGGTTTCCTGTAGTCTCTACCTTGGTTTTGAGTATATGGGA
   
Sequence Name 14-K125003-0022-079-B12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K125003-0022-079-B12C-8409
GCCATTTACAATTGAATTATCCTGGACTTGAGACCTTGGGTAGCAGCAAGACCAGAAAGA
TCATGCTCCATATACTCAAAAACAAGGTAGAGACTACAGGAAACCCGTGAAGTAACCAAA
CCTTGAAGCTTAATAACATTAGGATGATTAAGCCGTCGTAACACAAGAATCTCTCTAGCC
ATAAATTTAACACTCTCAGCTTCCAAATTATCAAACCTAACTTTCTTCAAAGCTACAATC
TTCCCACTCAATAAATCTTTCGCCTTGTACACATTGCTATACGTTCCTTGCCCAATCTAT
AACAATCAAAAACATTACTTCATTAAATTAAACCAAACATAAACTAATCAAAACAAAACA
AAACACTTTCTAATTATACCAAACCTAGTAATGATTATTACTTTATTAATGATTAGTGTT
TTTGTTACCTACCTTCTCGAGCTTTTCGTATGTGGTAGCTCTGCGAGGTGTTAAGTCTTT
GATCGAATCGCCACAAGCAGCAATAAGCCAAGGTGGCCATCCTTCCGGCGTACGGAGACT
CATCCCCGGACGGCGAGAAGAGCCGGCGGAGAAGTCGCCAGTGTGCCATGTTCTTCTCTC
CGGCTGAATCTGAATCGAATCTAAATCTTTCTTCTTCTTCTCTCCGATTTCGACAGAAAT
GGCGGCGGTGGTGGTTGAAGAAGAAGAAGGTAAATCCTCCGTATTACGGAGGTCTGCCGG
AGGAGGTTCATCGGGATTATTGTGTTTCCGTTTGTCTCCGGTGGATTCTTTGCATAAAAC
GCATCCCATCGGCTAAATGATTTTGAATCGGTGAAGCTTTCTCTCTTTAGCTTAACATTA
TCGCCGAAAGAGCTCAAAAATTGATTTTTTTCTCTTGGAGAGAGTGAGAATCGGATTTTG
TGTAAAACGACGGCGTTTTTGAGT

Last Updated on 06.12.2018 16:11