Sequences from confirmation amplicon


Confirmation sequences for line 079C09 and hit At1g22550 are shown.
Line ID 079C09
Locus At1g22550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7968086
Primers used for PCR CD92 - 8474, show primer details
   
Sequence Name 47-K106288-0022-079-C09C-8409
GenBank Accession LN506205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K106288-0022-079-C09C-8409
CGGACTGAGCCATTTAGATTTGAAGTAAGAGACAAGAACGTTATTACAAGTATGTAGATG
AGGGAAGCAACAACAATGGTACGGTAACGACCAAGATAAGCATCTGCAATGAAAGCTCCT
AAAACAGGAAGTATCGAGGCGGTTCCTGACCATGTGTTCACGTTCACGGCGGCGGTGGCT
GTTGATTGTCCGAGAGGTCCGGTGAGGTAAGTAATTAAGTTGGAACCAATCCCAAAGTAA
GCAAATCTTTCTCCAACTTCCACACCTAAAAAACCACACCAAAAAAATAAAATAAAATGT
TTAAAAAATAATGGTTTATGAGACAATTTACAAATCACAAAAACTGTTCTTTACCTATAA
TGTACCAGGCGGATCTCCATCCGCCGGTGGAAGATTTTCCGGCAGGAAGTCCACGGTGGT
CAACGGAGTCGCTGACGGAATCTTCTATA
   
Sequence Name 47-K106289-0022-079-C09C-CD92
GenBank Accession LN506206 [GenBank]
Sequencing primer gene specific primer, CD92: 5'-TTATAGAAGATTCCGTCAGCGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K106289-0022-079-C09C-CD92
CTGCCGGAATCTTCCCCGGCGGATGGAGATCCGCCTGGTACATTATAGGTAAAGAACAGT
TTTTGTGATTTGTAAATTGTCTCATAAACCATTATTTTTTAAACATTTTATTTTATTTTT
TTGGTGTGGTTTTTTAGGTGTGGAAGTTGGAGAAAGATTTGCTTACTTTGGGATTGGTTC
CAACTTAATTACTTACCTCACCGGACCTCTCGGACAATCAACAGCCACCGCCGCCGTGAA
CGTGAACACATGGTCAGGAACCGCCTCGATACTTCCTGTTTTAGGAGCTTTCATTGCAGA
TGCTTATCTTGGTCGTTACCGTACCATTGTTGTTGCTTCCCTCATCTACATACTTGTAAT
AACGTTCTTGTCTCTTACTTCAATATCTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATT
Primers used for PCR CD93 - 8474, show primer details
   
Sequence Name 90-K118835-0022-079-C09C-CD93
GenBank Accession LN515071 [GenBank]
Sequencing primer gene specific primer, CD93: 5'-TAAACCACCACAATAATCGACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K118835-0022-079-C09C-CD93
CAGACACCAATTGAAAACGATCCTCTACTTATTCTCTCCTTTGGATCTCCCACATCAAAC
TGGTCAGCACCAAAAGCTTGAACACATGGCTTGTGTCCGCCTTGTCCGATCGCCACCAAA
TACAGAGAACAAAAGAATAATATATTCACCCAAAAAAACGGTTTAGCAGAAGCGTTGCGG
TTATGTTGACGTTGCTCCGATAATCCCATTAGGATTAAGATAGAGGATAAGGTCAATAGT
CCTAGTCCCTGTAACAAAAATCCCCTCAAATTTTAGTGAAAATTCTAAAATGTTATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATTATAAT
   
Sequence Name 66-K118834-0022-079-C09C-8409
GenBank Accession LN511713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118834-0022-079-C09C-8409
TGAATATAACATTTTAGAATTTTCACTAAAATTTGAGGGGATTTTTGTTACAGGGACTAG
GACTATTGACCTTATCCTCTATCTTAATCCTAATGGGATTATCGGAGCAACGTCAACATA
ACCGCAACGCTTCTGCTAAACCGTTTTTTTGGGTGAATATATTATTCTTTTGTTCTCTGT
ATTTGGTGGCGATCGGACAAGGCGGACACAAGCCATGTGTTCAAGCTTTTGGTGCTGACC
AGTTTGATGTGGGAGATCCAAAGGAGAGAATAAGTAGAGGATCGTTTTTCAATTGGTGGT
TCTTGAGTTTATCCGCGGGAATAACTTTGTCGATTATTGTGGTGGTTTA

Last Updated on 06.12.2018 16:11