Sequences from confirmation amplicon


Confirmation sequences for line 079F05 and hit At4g33810 are shown.
Line ID 079F05
Locus At4g33810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16214538
Primers used for PCR 03dr - 8474, show primer details
   
Sequence Name 54-K110361-0022-079-F05C-03dr
GenBank Accession LN508511 [GenBank]
Sequencing primer gene specific primer, 03dr: 5'-ATTCATCAAATCGTTTGGATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K110361-0022-079-F05C-03dr
GGGTCTCCCACAACACAGTATGACCTCTAACCATATTCCATTCTCTTCCGCAAATTTTAA
CATTGAATCAGCTGCCGTGTAGTTCTCGTGACCACGTTCTTTCTCCGTTGTATACCATTT
CATTTCATTGGTGAAGGATGTGATTTTAAACCGCGACGCAAACCAATTTCTGTACCCTTC
GCTTTGTAGGATCCGGAAATTCATTGCGCAGCCTAATAGGAAAGATGGTTTGGTTTGTTC
TATAGATATCACTGCGCCTTTTACCGCAGTTTTGTTCTGATAAATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 38-K110360-0022-079-F05C-8409
GenBank Accession LN503018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K110360-0022-079-F05C-8409
TTGAATTTATCAGAACAAACTGCGGTAAAAGGCGCAGTGATATCTATAGAACAAACCAAA
CCATCTTTCCTATTAGGCTGCGCAATGAATTTCCGGATCCTACAAAGCGAAGGGTACAGA
AATTGGTTTGCGTCGCGGTTTAAAATCACATCCTTCACCAATGAAATGAAATGGTATACA
ACGGAGAAAGAACGTGGTCACGAGAACTACACGGCAGCTGATTCAATGTTAAAATTTGCG
GAAGAGAATGGAATATTGGTTAGAGGTCATACTGTGTTGTGGGATGACCCTTTAATGCAG
CCAACTTGGGTGCCAAAAATAGAAGATCCAAACGATTTGATGA

Last Updated on 06.12.2018 16:11