Sequences from confirmation amplicon


Confirmation sequences for line 079G10 and hit At1g61390 are shown.
Line ID 079G10
Locus At1g61390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22652167
Primers used for PCR RK48 - 8474, show primer details
   
Sequence Name 01-K016275-022-079-G10C-8409
GenBank Accession LN486365 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K016275-022-079-G10C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCAGCCTATCTATTTTCGT
AATCTTGGTCTTTGCCGCTTATAAGTCCTGGAGATACAGAACAAAACAAAATGGTGAGAC
ACAGGCACTTTGTTCTCACTCACTCTCTTTCCTTGTCCTCTTTTTTAATCTAACTCGGTA
CCAAATCCGAAGCAAATTAATCTCTTCCTCTTCATCTGATACACACTACATTTCTAGAAT
TCTTAAGTTGTGTCCCAAGGTACAGTCTCAGGTTGACAGTTCTTTTGGGAAGTCTATATG
GTCAGTTTAAAGCTAGATGTCCTAACGGTTAATATGTTCGCTTCTATTGAAAGTCAATGC
TTATGCTAATAAACTGCTACAAATTAAGCACTAATACATGCACACTTGTTACTCGTAGAA
CCAAATCCTATGTTCATCCACAGTTCACAAGATGCATGGGCGAAGGATATGGAA
   
Sequence Name 02-K016275-022-079-G10C-RK48
GenBank Accession LN486815 [GenBank]
Sequencing primer gene specific primer, RK48: 5'-GACATCTTGTGGTTCCATATCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K016275-022-079-G10C-rk48
CATCTTGTGAACTGTGGATGAACATAGGATTTGGTTCTACGAGTAACAAGTGTGCATGTA
TTAGTGCTTAATTTGTAGCAGTTTATTAGCATAAGCATTGACTTTCAATAGAAGCGAACA
TATTAACCGTTAGGACATCTAGCTTTAAACTGACCATATAGACTTCCCAAAAGAACTGTC
AACCTGAGACTGTACCTTGGGACACAACTTAAGAATTCTAGAAATGTAGTGTGTATCAGA
TGAAGAGGAAGAGATTAATTTGCTTCGGATTTGGTACCGAGTTAGATTAAAAAAGAGGAC
AAGGAAAGAGAGTGAGTGAGAACAAAGTGCCTGTGTCTCACCATTTTGTTTTGTTCTGTA
TCTCCAGGACTTATAAGCGGCAAAGACCAAGATTACGAAAATAGATAGGCTGACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTGCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACAT

Last Updated on 06.12.2018 16:11