Sequences from confirmation amplicon


Confirmation sequences for line 080C04 and hit At5g42240 are shown.
Line ID 080C04
Locus At5g42240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16889528
Primers used for PCR 03xp - 8474, show primer details
   
Sequence Name 42-K119512-0022-080-C04C-03xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 03xp: 5'-TTAATGGTGTAGGCAAACTACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K119512-0022-080-C04C-03xp
TCTTGAGCAAGTTCTCTTACGAGAGTTCTTGAACCCCCGAATGGAACTACAGAATCTTGA
TCTCCACTACAATATCGACAATGCAATTAAGACATGAACAGAAGGAGATAAAATGGATCT
TTAATAGATGTATAACCTGAAGATCCAAATAGGAGTTTTGTTCAGTATAATTCTCTTCAG
AATCGGAAGCATGTCGATGTTCCCATCGATATCAGAGTAGTTTAACACGCTGTGACAGGC
AAAGTGAACAGCCAAAAGCAATCAATGTAGAGATATATGAACTCCAGTTATAAATAGCTT
CTCTAATTTTGTGATCAATAAAACCGAGAGAAAGTACGATAATTTACCCGCTGCACATTG
ACCATGAATAAGGCAGATGAGTACGGTTCGCATGAAGCGCCTTTTGTACCTCTGGAAGAT
TAAAGTAGAACCGTCTTTCATAGGTCATACACACATCCACTCCCATGCTCATTTTAGTAG
CCTGCAGTTGTTTTAGTAAATTGACGTATATTTCAACAAAAATTTTGTTGGTGGAACAAA
AGATAAAAAAGGGCTGACCATTTTCTTGAGCCTCAGCTCTTGCTGGACGATAGACGGATA
ACATACATCGAGGAGAACATCATAGTTGTTGACATACTCAGTGATAATGTTTTCAGTTTC
ACTAATAGCTTCATTGCAGGCCGTGCTTACGTTATGGGGGCTAGCAAATGTGTAATCATC
AAAGTCACATTGACTCGTGATTGTGAGTTTTAGTTCATCAGAGATCATGCCATGCGACCA
GAAGAACTCGTAGGTAGCTGGACTGTCTCTGTCAAGCTTCAGAAGTGGATTTCCAATCTG
ATCAGAAAAAATAAGACAAAGGAATTCGGAGGGGAACAAAAGAGTTAATGGAATGCAAGA
AGAAAGGTTCTTT
   
Sequence Name 10-K119511-0022-080-C04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119511-0022-080-C04C-8409
CATTGCTGATCTTGAATGTAACCTTAAAACATCATCACTATTATATATACATATATATAT
ATATATTTTTTTTTTTTACGGGACATTACATACCTCAACGGGGTGATGCGATTCTCAGCT
ACAATTCACACTCAAGGGGATTCAAATTCAACATCAAAGGCGTTGCTGTAAGCAAAAAAA
CCTTTCTTCTTGCATTCCATTAACTCTTTTGTTTCCCTTCAGAATTCTTTTGTCTTATTT
TTTTCTGATCAGATGGGGAATCCACTTCTGAAGCTTGACAGAAACAGTCCACCTCCCTAC
GATTTCTTCTGGTCGCAGGGCATGATCTCTGATGAACTAAAACTCACAATCACGAGTCAA
TGTGACTTTGATGATTACACATTTGCTACCCCCCATAACGTAAGCACGGCCTGCAATGAA
GCTTTTAGTGAAACTGAAAACTTTATCACTGAGTATGTCAACAACTATGATGTTCCCCCC
GATGTATGTTATCCGTCTATCGCCCAGCAAGAGCTGAGGCTCAAAAAAATGGTCACCCCT
TTTTTATCTTTTGTTCCCCCAACAAAATTTTTGTTGAAATATACGTCAATTTACAAAAAC
AACTGCGGGCTACTAAAATGAGCATGGGAGTGGATGTGTGTATGACCTATAAAAGACGGT
TCTACTTTAATCTTCCAGAGGTACAAAAGGCGCTTCATGCGAACCGTACTCATCTGCCTT
ATTCATGGTCAATGTGCAGCGGGAAAATTATCGTACTTTCTCTCGGTTTTATTGATCACA
AAATTAGAGAAGCTATTTATAACGGGAGTTCATATATCTCTACATTGATTGCTTTTGGCT
GTTCACTTTGCCTGTCACAGC

Last Updated on 06.12.2018 16:11