Sequences from confirmation amplicon


Confirmation sequences for line 080C05 and hit At4g31780 are shown.
Line ID 080C05
Locus At4g31780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15375929
Primers used for PCR IW49 - 8474, show primer details
   
Sequence Name 46-K033213-022-080-C05C-IW49
GenBank Accession LN505779 [GenBank]
Sequencing primer gene specific primer, IW49: 5'-TCCATTTTCGTTATAAACCCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033213-022-080-C05C-IW49
GACTAAATCAAACGCCTAAGATTGTGGATTTGGTATGAAGAGTGACAAATGTAGAGTACC
TGGACAGGGATTTTCCAGTCTAAGGAACTTAATTTGCTTTGGAGTTTCTTGTTCCGTCCA
CAGATTATAAGAACCTGACCTACTGCTTCACCAAGATTCTTATCATACAAAGCATCTGCA
AGTGCTCTTGCTGTTGCCTCGATGGGACCCATCCCTTCTCCTCCACCCATTAACAAAACA
GCTGGAAGATTCTCATCCATCCCTAGCTCCCTTCTTAATTCAACCTGCGATTTTTTCGGA
CAGATACTATTACATTTGGATGAGTCGGAAGCAACAAAGAACAGCTAGGAATGATTCCAA
AACCGACCTTTGGGCGAACTGGTTTCACAAAGGAAGGTCGAACAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CA
   
Sequence Name 38-K033213-022-080-C05C-8409
GenBank Accession LN502892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K033213-022-080-C05C-8409
TTACAATTGAATATATCCTGTTCGACCTTCCTTTGTGAAACCAGTTCGCCCAAAGGTCGG
TTTTGGAATCATTCCTAGCTGTTCTTTGTTGCTTCCGACTCATCCAAATGTAATAGTATC
TGTCCGAAAAAATCGCAGGTTGAATTAAGAAGGGAGCTAGGGATGGATGAGAATCTTCCA
GCTGTTTTGTTAATGGGTGGAGGAGAAGGGATGGGTCCCATCGAGGCAACAGCAAGAGCA
CTTGCAGATGCTTTGTATGATAAGAATCTTGGTGAAGCAGTAGGTCAGGTTCTTATAATC
TGTGGACGGAACAAGAAACTCCAAAGCAAATTAAGTTCCTTAGACTGGAAAATCCCTGTC
CAGGTACTCTACATTTGTCACTCTTCATACCAAATCCACAATCTTAGGCGTTTGATGTTA
GTCACTGAAACTGTGTTCTTTATGTCATAGGTTAAGGGGTTTATAACGAAAATGG

Last Updated on 06.12.2018 16:11