Sequences from confirmation amplicon


Confirmation sequences for line 080E06 and hit At1g02640 are shown.
Line ID 080E06
Locus At1g02640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:564387
Primers used for PCR CL22 - 8474, show primer details
   
Sequence Name 56-K028749-022-080-E06C-8409
GenBank Accession LN508995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K028749-022-080-E06C-8409
GCGATACAATGGACGCAGCAACAGCTACACTGAGATTCTGCCAGCTTTCAGTTCCTATAC
CGGAGAGAGTCAGAGATTTGATCGGACGGTTGACATTGGCCGAGAAAGTGAGCTTGTTAG
GGAACACTGCGGCGGCGATACCACGTCTAGGAATCAAAGGGTACGAGTGGTGGTCGGAGG
CTTTACACGGCGTTTCAAATGTGGGACCCGGTACTAAGTTCGGTGGGGTTTACCCTGCAG
CCACCAGTTTCCCTCAAGTCATCACCACCGTTGCTTCTTTCAATGCCTCCTTGTGGGAAT
CCATCGGACGGGTCAGATTATTTCTTTCTCTCTCTTTTATCCATTAAGTTAAAAACACCT
ATTTCAATTATTTTTGTGTGTGCCCCCGATCTAACTACTAAATACTTTTCACTATTTTCT
CTGTGAAAATGACGTAAGTAATAATGTTAATTATATATATATACAGCTCACATTTATTAT
GTATTTAATTACCTCTAGTGCGATTTATATGTAAAACACTTTTACTGTATCGAAATATTC
TCTTTTATCGGAACTAATATTCGTTTTTTGTTTTTTATTTTTAAAATAACTTATATACAA
TTGTGATATTTCGACACTTCTCATGTTCTGATTTTGTCCGGTACTTCATGCAAATAAACA
TACGCACG
   
Sequence Name 56-K028749-022-080-E06C-CL22
GenBank Accession LN508996 [GenBank]
Sequencing primer gene specific primer, CL22: 5'-AGGCTAATAAGAGATTCGTGCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K028749-022-080-E06C-CL22
ACATGAGAAGTGTCGAAATATCACAATTGTATATAAGTTATTTTAAAAATAAAAAACAAA
AAACGAATATTAGTTCCGATAAAAGAGAATATTTCGATACAGTAAAAGTGTTTTACATAT
AAATCGCACTAGAGGTAATTAAATACATAATAAATGTGAGCTGTATATATATATAATTAA
CATTATTACTTACGTCATTTTCACAGAGAAAATAGTGAAAAGTATTTAGTAGTTAGATCG
GGGGCACACACAAAAATAATTGAAATAGGTGTTTTTAACTTAATGGATAAAAGAGAGAGA
AAGAAATAATCTGACCCGTCCGATGGATTCCCACAAGGAGGCATTGAAAGAAGCAACGGT
GGTGATGACTTGAGGGAAACTGGTGGCTGCAGGGTAAACCCCACCGAACTTAGTACCGGG
TCCCACATTTGAAACGCCGTGTAAAGCCTCCGACCACCACTCGTACCCTTTGATTCCTAG
ACGTGGTATCGCCGCCGCAGTGTTCCCTAACAAGCTCACTTTCTCGGCCAATGTCAACCG
TCCGATCAAATCTCTGACTCTCTCCGGTATAGGAACTGAAAGCTGGCAGAATCTCAGTGT
AGCTGTTGCTGCGTCCTTTGTATCGCAAGCAAACGTTTCACGGCTATGAACGCAAACGCT
GCTGCTGCTTATCAAGAAGAAGAGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATTAAACG
ACAAATTACGATC

Last Updated on 06.12.2018 16:11